HPLC-based metabolic profiling and quality control of leaves of different Panax species.
HPLC-based metabolic profiling and quality control of leaves of different Panax species.
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DOI：
10.5142/jgr.2013.37.248
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发表时间：
2013-04
影响因子：
6.3
通讯作者：
Shin YS
中科院分区：
文献类型：
--
作者：
Yang SO;Lee SW;Kim YO;Sohn SH;Kim YC;Hyun DY;Hong YP;Shin YS
在韩国海南省收获来自人参Meyer（韩国人参和中国人参）和西洋参（西洋参）的叶，并使用基于HPLC的代谢谱进行分析以研究主要代谢物的模式。采用偏最小二乘判别分析（PLS-DA）对HPLC色谱数据进行分析。在PLS-DA评分图中，来自不同国家的人参种和/或来源之间存在明显的分离。朝鲜人参叶片中的Rg 1、Re、Rg 2、Rb 2、Rb 3和Rd的含量高于中国人参和西洋参，西洋参叶片中的Rb 1含量高于朝鲜人参和中国人参。HPLC色谱数据与多元统计分析相结合，可用于人参代谢物含量的分析，并进行质量控制。
Leaves from Panax ginseng Meyer (Korean origin and Chinese origin of Korean ginseng) and P. quinquefolius (American ginseng) were harvested in Haenam province, Korea, and were analyzed to investigate patterns in major metabolites using HPLC-based metabolic profiling. Partial least squares discriminant analysis (PLS-DA) was used to analyze the HPLC chromatogram data. There was a clear separation between Panax species and/or origins from different countries in the PLS-DA score plots. The ginsenoside compounds of Rg1, Re, Rg2, Rb2, Rb3, and Rd in Korean leaves were higher than in Chinese and American ginseng leaves, and the Rb1 level in P. quinquefolius leaves was higher than in P. ginseng (Korean origin or Chinese origin). HPLC chromatogram data coupled with multivariate statistical analysis can be used to profile the metabolite content and undertake quality control of Panax products.
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