Streamlined Synthesis and Assembly of a Hybrid Sensing Architecture with Solid Binding Proteins and Click Chemistry.
Streamlined Synthesis and Assembly of a Hybrid Sensing Architecture with Solid Binding Proteins and Click Chemistry.
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DOI：
10.1021/jacs.7b00519
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发表时间：
2017-03-22
影响因子：
15
通讯作者：
Baneyx F
中科院分区：
文献类型：
--
作者：
Swift BJ;Shadish JA;DeForest CA;Baneyx F
结合生物正交化学，利用黏合剂和形态发生固体结合肽工程蛋白合成具有生命系统经济性和高效性的杂化材料是一条很有前途的途径。利用氯霉素的光学传感作为概念证明，我们在这里展示了一种由硫化锌和硅结合肽在其β-桶的两侧进行工程改造的GFP变体，支持蛋白质覆盖的ZnS:Mn纳米晶体的制造，这种纳米晶体表现出有机和无机荧光团的联合发射特征。通过菌株促进叠氮化物-炔环加成将氯霉素特异性DNA适配体偶联到蛋白质外壳上，并通过二氧化硅结合序列介导的纳米结构自发浓度到二氧化硅颗粒上，通过分析物介导的亚330 nm激发光的内过滤，可以直观检测环境和临床相关的氯霉素浓度。
Combining bioorthogonal chemistry with the use of proteins engineered with adhesive and morphogenetic solid-binding peptides is a promising route for synthesizing hybrid materials with the economy and efficiency of living systems. Using optical sensing of chloramphenicol as a proof of concept, we show here that a GFP variant engineered with zinc sulfide and silica-binding peptides on opposite sides of its β-barrel supports the fabrication of protein-capped ZnS:Mn nanocrystals that exhibit the combined emission signatures of organic and inorganic fluorophores. Conjugation of a chloramphenicol-specific DNA aptamer to the protein shell through strain-promoted azide–alkyne cycloaddition and spontaneous concentration of the resulting nanostructures onto SiO2 particles mediated by the silica-binding sequence enables visual detection of environmentally and clinically relevant concentrations of chloramphenicol through analyte-mediated inner filtering of sub-330 nm excitation light.
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