Platelets enhance malignant behaviours of gastric cancer cells via direct contacts.
Platelets enhance malignant behaviours of gastric cancer cells via direct contacts.
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DOI：
10.1038/s41416-020-01134-7
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发表时间：
2021-03
影响因子：
8.8
通讯作者：
Ichikawa D
中科院分区：
文献类型：
--
作者：
Saito R;Shoda K;Maruyama S;Yamamoto A;Takiguchi K;Furuya S;Hosomura N;Akaike H;Kawaguchi Y;Amemiya H;Kawaida H;Sudo M;Inoue S;Kono H;Suzuki-Inoue K;Ichikawa D
In this study, we aimed to analyse human cancer cell–platelet interactions in functional cell analyses and explore the molecular mechanisms behind tumour progression. Various functional analyses of gastric cancer (GC) cells were performed after direct/indirect co-incubation with platelets derived from GC patients.在与直接和间接 GC 细胞-血小板接触共培养后，进行了进一步详细的表达和信号分析。 Malignant behaviours of cancer cells, such as proliferation, migration, invasion and adhesion, were significantly enhanced after direct co-incubation with platelets.微阵列分析表明多个基因发生变化，包括上皮间质转化（EMT）相关基因。 Among them, matrix metalloproteinase 9 was notably upregulated, which was validated by quantitative reverse transcription–polymerase chain reaction and western blot.此外，只有在与血小板直接共孵育后才能观察到这种变化。 This study demonstrated that platelets from GC patients promote malignant behaviours of GC cells through EMT-related signalling, especially by direct contact with tumour cells.
In this study, we aimed to analyse human cancer cell–platelet interactions in functional cell analyses and explore the molecular mechanisms behind tumour progression. Various functional analyses of gastric cancer (GC) cells were performed after direct/indirect co-incubation with platelets derived from GC patients. Further detailed expression and signalling analyses were performed after co-culture with direct and indirect GC cells–platelet contact. Malignant behaviours of cancer cells, such as proliferation, migration, invasion and adhesion, were significantly enhanced after direct co-incubation with platelets. Microarray analyses demonstrated changes in multiple genes, including epithelial–mesenchymal transition (EMT)-related genes. Among them, matrix metalloproteinase 9 was notably upregulated, which was validated by quantitative reverse transcription–polymerase chain reaction and western blot. Further, this change was only observed after direct co-incubation with platelets. This study demonstrated that platelets from GC patients promote malignant behaviours of GC cells through EMT-related signalling, especially by direct contact with tumour cells.
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