Surface enhanced Raman spectroscopy for microfluidic pillar arrayed separation chips.
Surface enhanced Raman spectroscopy for microfluidic pillar arrayed separation chips.
复制标题
用于微流控柱阵列分离芯片的表面增强拉曼光谱。
DOI：
10.1039/c2an16239c
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
M. Sepaniak
中科院分区：
文献类型：
--
作者：
L. C. Taylor;Teresa B Kirchner;N. Lavrik;M. Sepaniak
许多研究已经解决了实施用于化学和生物分离应用的小型化微流体平台的挑战。然而，能够在连续的超低体积流状态下提供有价值的样品信息的真实的时间检测方案的集成尚未完全解决。在这份报告中，我们提出了一种基于芯片的色谱系统，包括柱阵列分离柱，然后是一个试剂通道，用于被动混合的银胶体溶液进入洗脱液流，使表面增强拉曼光谱（Sers）检测。我们的设计是第一个集成芯片为基础的微流控装置，结合联合收割机压力驱动的分离能力与真实的时间Sers检测。通过这种方法，我们证明了收集独特Sers光谱的能力，或没有完整的分辨率的色谱带。计算流体动力学（CFD）模拟用于模拟微流体通道中的两个汇合流的扩散混合行为和速度分布。我们评估的Sers光谱带强度和色谱效率的模型分析物的动力学因素，以及信号采集速率。此外，我们讨论了使用普朗尼克改性的银胶体溶液作为消除污染的手段，通常由纳米颗粒粘附到通道表面。
Numerous studies have addressed the challenges of implementing miniaturized microfluidic platforms for chemical and biological separation applications. However, the integration of real time detection schemes capable of providing valuable sample information under continuous, ultra low volume flow regimes has not fully been addressed. In this report we present a chip based chromatography system comprising of a pillar array separation column followed by a reagent channel for passive mixing of a silver colloidal solution into the eluent stream to enable surface enhanced Raman spectroscopy (SERS) detection. Our design is the first integrated chip based microfluidic device to combine pressure driven separation capability with real time SERS detection. With this approach we demonstrate the ability to collect distinctive SERS spectra with or without complete resolution of chromatographic bands. Computational fluidic dynamic (CFD) simulations are used to model the diffusive mixing behaviour and velocity profiles of the two confluent streams in the microfluidic channels. We evaluate the SERS spectral band intensity and chromatographic efficiency of model analytes with respect to kinetic factors as well as signal acquisition rates. Additionally, we discuss the use of a pluronic modified silver colloidal solution as a means of eliminating contamination generally caused by nanoparticle adhesion to channel surfaces.
影响因子：
7.4
作者：
Chambers, Andrew G.;Mellors, J. Scott;Henley, W. Hampton;Ramsey, J. Michael
通讯作者：
Ramsey, J. Michael
影响因子：
7.4
作者：
Liu, Jikun;White, Ian;DeVoe, Don L.
通讯作者：
DeVoe, Don L.
影响因子：
7.4
作者：
Xie, J;Miao, YN;Lee, TD
通讯作者：
Lee, TD