Monolithic integration of two-dimensional liquid chromatography-capillary electrophoresis and electrospray ionization on a microfluidic device.
Monolithic integration of two-dimensional liquid chromatography-capillary electrophoresis and electrospray ionization on a microfluidic device.
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DOI：
10.1021/ac102437z
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发表时间：
2011-02-01
影响因子：
7.4
通讯作者：
Ramsey, J. Michael
中科院分区：
文献类型：
--
作者：
Chambers, Andrew G.;Mellors, J. Scott;Henley, W. Hampton;Ramsey, J. Michael
描述了一种能够进行二维反相液相色谱-毛细管电泳和集成电喷雾电离 (LC-CE-ESI) 的微流体装置，用于基于质谱 (MS) 的蛋白质组学应用。传统仪器用于 LC 样品注射和 LC 流动相的输送。该玻璃微加工装置包含一个样品捕获区域和一个填充有反相颗粒的液晶通道。在跨通道交叉点处将 LC 流出物快速电动注射到 CE 尺寸中。 CE 分离通道终止于方形装置的一角，该装置充当集成电喷雾尖端。除了 LC-CE-ESI 之外，该设备还用于 LC-ESI，无需对仪器进行任何修改。为了评估该系统，对蛋白质消化物进行了 LC-MS 和 LC-CE-MS 分析并进行了比较。
A microfluidic device capable of two-dimensional reversed-phase liquid chromatography-capillary electrophoresis with integrated electrospray ionization (LC-CE-ESI) for mass spectrometry (MS)-based proteomic applications is described. Traditional instrumentation was used for the LC sample injection and delivery of the LC mobile phase. The glass microfabricated device incorporated a sample-trapping region and an LC channel packed with reversed-phase particles. Rapid electrokinetic injections of the LC effluent into the CE dimension were performed at a cross channel intersection. The CE separation channel terminated at a corner of the square device, which functioned as an integrated electrospray tip. In addition to LC-CE-ESI, this device was used for LC-ESI without any instrumental modifications. To evaluate the system, LC-MS and LC-CE-MS analysis of protein digests were performed and compared.
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影响因子：
7.4
作者：
Mellors, J. Scott;Jorabchi, Kaveh;Smith, Lloyd M.;Ramsey, J. Michael
通讯作者：
Ramsey, J. Michael
影响因子：
7.4
作者：
Mellors, J. S.;Gorbounov, V.;Ramsey, R. S.;Ramsey, J. M.
通讯作者：
Ramsey, J. M.
影响因子：
7.4
作者：
Bergström, SK;Samskog, J;Markides, KE
通讯作者：
Markides, KE
影响因子：
4.1
作者：
Lord, GA;Gordon, DB;King, BW
通讯作者：
King, BW
影响因子：
4.1
作者：
Lin, YH;Lee, GB;Chen, SH
通讯作者：
Chen, SH