Day/night variations of high-molecular-weight adiponectin and lipocalin-2 in healthy men studied under fed and fasted conditions.
Day/night variations of high-molecular-weight adiponectin and lipocalin-2 in healthy men studied under fed and fasted conditions.
复制标题
DOI：
10.1007/s00125-010-1869-7
复制
发表时间：
2010-11
期刊：
影响因子：
8.2
通讯作者：
Mantzoros, C. S.
中科院分区：
文献类型：
--
作者：
Scheer, F. A. J. L.;Chan, J. L.;Fargnoli, J.;Chamberland, J.;Arampatzi, K.;Shea, S. A.;Blackburn, G. L.;Mantzoros, C. S.
关键词：
脂联素和脂质运载蛋白-2是脂肪细胞来源的血浆蛋白，已被提出对胰岛素敏感性具有相反的作用。鉴于睡眠、昼夜节律计时系统和葡萄糖代谢之间的流行病学、生理学和分子联系，本研究的目的是评估睡眠/觉醒周期和禁食/进食周期对高分子量脂联素（HMW-脂联素;生物活性形式）和脂质运载蛋白-2的影响。我们还旨在比较这些蛋白质水平的24小时节律与皮质醇，瘦素，瘦素结合蛋白和总脂联素。瘦型男性在进食状态（n=8，年龄：20.9±2.1岁，BMI：22.8±2.3 kg/m2）或空腹状态（空腹3天，n=4，25.3±3.9岁，BMI：23.3±2.2 kg/m2）下进行了为期3天的实验室研究。睡眠事件安排在23：00至07：00的黑暗中进行。在每项研究的第三天，每15分钟采集一次血样，持续24小时。进食时，HMW-脂联素和脂质运载蛋白-2具有较大的日节律，在夜间出现谷值（HMW-脂联素：约04：00小时，峰谷振幅36%，p<0.0001;脂质运载蛋白-2：约04：00小时，40%，p<0.0001）。禁食第3天，时间和相对振幅不变（HMW-脂联素：约04：00小时，38%，p=0.0014;脂质运载蛋白-2：约05：00小时，38%，p=0.0043）。这些数据表明，HMW-脂联素和脂质运载蛋白-2都具有显著的日/夜节律，都在夜间具有谷值，这些不是由进食/禁食周期驱动的，并且重要的是报告和/或记录用于此类测定的一天中的时间。需要进一步的研究来确定HMW-脂联素水平的日节律是否影响胰岛素敏感性的日节律。ClinicalTrials.gov NCT00140205。
Adiponectin and lipocalin-2 are adipocyte-derived plasma proteins that have been proposed to have opposite effects on insulin sensitivity. Given the epidemiological, physiological and molecular links between sleep, the circadian timing system and glucose metabolism, the aim of this study was to assess effects of the sleep/wake cycle and the fasting/feeding cycle on high-molecular-weight adiponectin (HMW-adiponectin; the biologically active form) and lipocalin-2. We also aimed to compare the 24 h rhythms in the levels of these proteins with those of cortisol, leptin, leptin-binding protein and total adiponectin. Lean men underwent a 3 day in-laboratory study, either in the fed state (n=8, age: 20.9±2.1 years, BMI: 22.8±2.3 kg/m2) or fasting state (3 day fast, n=4, 25.3±3.9 years, BMI: 23.3±2.2 kg/m2). The sleep episode was scheduled in darkness from 23:00 to 07:00 hours. Blood was sampled every 15 min for 24 h on the third day of each study. While fed, HMW-adiponectin and lipocalin-2 had large daily rhythms with troughs at night (HMW-adiponectin: ~04:00 hours, peak-to-trough amplitude 36%, p<0.0001; lipocalin-2: ~04:00 hours, 40%, p<0.0001). On the third day of fasting, the timing and relative amplitudes were unchanged (HMW-adiponectin: ~04:00 hours, 38%, p=0.0014; lipocalin-2: ~05:00 hours, 38%, p=0.0043). These data show that HMW-adiponectin and lipocalin-2 both have significant day/night rhythms, both with troughs at night, that these are not driven by the feeding/fasting cycle, and that it is important to report and/or standardise the time of day for such assays. Further studies are required to determine whether the daily rhythm of HMW-adiponectin levels influences the daily rhythm of insulin sensitivity. ClinicalTrials.gov NCT00140205.
登录
查看更多内容
DOI：
10.1073/pnas.0808180106
发表时间：
2009-03-17
影响因子：
11.1
作者：
Scheer, Frank A. J. L.;Hilton, Michael F.;Shea, Steven A.
通讯作者：
Shea, Steven A.
影响因子：
15.9
作者：
Chan, JL;Heist, K;Mantzoros, CS
通讯作者：
Mantzoros, CS
影响因子：
7.7
作者：
Abbasi, F;Chu, JW;Reaven, PD
通讯作者：
Reaven, PD
影响因子：
7.7
作者：
Law IK;Xu A;Lam KS;Berger T;Mak TW;Vanhoutte PM;Liu JT;Sweeney G;Zhou M;Yang B;Wang Y
通讯作者：
Wang Y
DOI：
10.1038/nrendo.2009.23
发表时间：
2009-05
期刊：
Nature reviews. Endocrinology
影响因子：
--
作者：
通讯作者：
--