Non-invasive quantification of the mitochondrial redox state in livers during machine perfusion.
Non-invasive quantification of the mitochondrial redox state in livers during machine perfusion.
复制标题
机器灌注过程中肝脏中线粒体氧化还原状态的非侵入性定量。
DOI：
10.1371/journal.pone.0258833
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发表时间：
2021
期刊：
影响因子：
3.7
通讯作者：
Tessier SN
中科院分区：
文献类型：
--
作者：
de Vries RJ;Cronin SEJ;Romfh P;Pendexter CA;Jain R;Wilks BT;Raigani S;van Gulik TM;Chen P;Yeh H;Uygun K;Tessier SN
Ischemia reperfusion injury (IRI) is a critical problem in liver transplantation that can lead to life-threatening complications and substantially limit the utilization of livers for transplantation. However, because there are no early diagnostics available, fulminant injury may only become evident post-transplant. Mitochondria play a central role in IRI and are an ideal diagnostic target. During ischemia, changes in the mitochondrial redox state form the first link in the chain of events that lead to IRI. In this study we used resonance Raman spectroscopy to provide a rapid, non-invasive, and label-free diagnostic for quantification of the hepatic mitochondrial redox status. We show this diagnostic can be used to significantly distinguish transplantable versus non-transplantable ischemically injured rat livers during oxygenated machine perfusion and demonstrate spatial differences in the response of mitochondrial redox to ischemia reperfusion. This novel diagnostic may be used in the future to predict the viability of human livers for transplantation and as a tool to better understand the mechanisms of hepatic IRI.
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影响因子：
2.2
作者：
Bruinsma BG;Uygun K
通讯作者：
Uygun K
影响因子：
3.7
作者：
Brazhe NA;Treiman M;Faricelli B;Vestergaard JH;Sosnovtseva O
通讯作者：
Sosnovtseva O
DOI：
10.1111/ajt.12727
发表时间：
2014-06
期刊：
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons
影响因子：
--
作者：
Bruinsma BG;Yeh H;Ozer S;Martins PN;Farmer A;Wu W;Saeidi N;Op den Dries S;Berendsen TA;Smith RN;Markmann JF;Porte RJ;Yarmush ML;Uygun K;Izamis ML
通讯作者：
Izamis ML
影响因子：
15.3
作者：
Cannistra, Marco;Ruggiero, Michele;Nardo, Bruno
通讯作者：
Nardo, Bruno
影响因子：
46.9
作者：
de Vries, Reinier J.;Tessier, Shannon N.;Uygun, Korkut
通讯作者：
Uygun, Korkut