Fate-restricted retinal progenitor cells adopt a molecular profile and spatial position distinct from multipotent progenitor cells.
Fate-restricted retinal progenitor cells adopt a molecular profile and spatial position distinct from multipotent progenitor cells.
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DOI：
10.1016/j.ydbio.2018.06.023
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发表时间：
2018-11-01
影响因子：
2.7
通讯作者：
Emerson MM
中科院分区：
文献类型：
--
作者：
Buenaventura DF;Ghinia-Tegla MG;Emerson MM
在发育过程中，多能视网膜祖细胞产生大量独特的细胞类型。最近的证据表明，除了多能性祖细胞外，还有命运限制的祖细胞状态。在这里，我们报告了一个转录组分析的命运限制祖细胞偏向于产生视锥细胞和水平细胞，标记的THRB顺式调节元件ThrbCRM 1。与富含多能祖细胞的对照群体相比，鉴定了几种被认为是泛祖细胞的基因，如VSX 2、LHX 2和PAX 6，在这些命运限制性视网膜祖细胞中下调。这种差异调节发生在小鸡和小鼠中不同的限制祖细胞群体中，这表明这是视网膜发育过程中祖细胞动力学的保守特征。S-期标记还显示，核位置的限制祖细胞群体占据不同的空间小生境内的发育鸡视网膜。使用接近VSX 2基因的保守调控元件，鉴定了来自在锥/水平细胞祖细胞中富集的特异性转录因子的潜在负反馈机制。本研究鉴定了从多能视网膜祖细胞生成命运限制性视网膜祖细胞过程中发生的保守分子和细胞变化。
During development, multipotent retinal progenitor cells generate a large number of unique cell types. Recent evidence suggests that there are fate-restricted progenitor cell states in addition to multipotent ones. Here we report a transcriptomic analysis of fate-restricted progenitor cells biased to produce cone photoreceptors and horizontal cells, marked by the THRB cis-regulatory element ThrbCRM1. Comparison to a control population enriched in multipotent progenitor cells identified several genes considered to be pan-progenitor, such as VSX2, LHX2, and PAX6, as downregulated in these fate-restricted retinal progenitor cells. This differential regulation occurs in chick and in a different restricted progenitor population in mouse suggesting that this is a conserved feature of progenitor dynamics during retinal development. S-phase labeling also revealed that nuclear positions of restricted progenitor populations occupy distinct spatial niches within the developing chick retina. Using a conserved regulatory element proximal to the VSX2 gene, a potential negative feedback mechanism from specific transcription factors enriched in cone/horizontal cell progenitor cells was identified. This study identifies conserved molecular and cellular changes that occur during the generation of fate restricted retinal progenitor cells from multipotent retinal progenitor cells.
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影响因子：
16.2
作者：
HOLT, CE;BERTSCH, TW;HARRIS, WA
通讯作者：
HARRIS, WA
影响因子：
4.6
作者：
Edqvist, PHD;Hallböök, F
通讯作者：
Hallböök, F
影响因子：
2.7
作者：
Brzezinski JA 4th;Prasov L;Glaser T
通讯作者：
Glaser T
在哺乳动物夜间视觉演变过程中，从短波长敏感锥中募集杆感光体。
影响因子：
11.8
作者：
Kim JW;Yang HJ;Oel AP;Brooks MJ;Jia L;Plachetzki DC;Li W;Allison WT;Swaroop A
通讯作者：
Swaroop A
影响因子：
5.3
作者：
Baye, Lisa M.;Link, Brian A.
通讯作者：
Link, Brian A.