Plasmodium falciparum Gametocyte Density and Infectivity in Peripheral Blood and Skin Tissue of Naturally Infected Parasite Carriers in Burkina Faso.
Plasmodium falciparum Gametocyte Density and Infectivity in Peripheral Blood and Skin Tissue of Naturally Infected Parasite Carriers in Burkina Faso.
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DOI：
10.1093/infdis/jiz680
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发表时间：
2021-05-28
期刊：
影响因子：
--
通讯作者：
Bousema T
中科院分区：
文献类型：
--
作者：
Meibalan E;Barry A;Gibbins MP;Awandu S;Meerstein-Kessel L;Achcar F;Bopp S;Moxon C;Diarra A;Debe S;Ouédraogo N;Barry-Some I;Badoum ES;Fagnima T;Lanke K;Gonçalves BP;Bradley J;Wirth D;Drakeley C;Guelbeogo WM;Tiono AB;Marti M;Bousema T
恶性疟原虫的传播依赖于成熟的配子体细胞，这些配子体细胞可以被蚊子在人类皮肤上吸食血液时摄入。尽管配子细胞皮肤隔离长期以来一直被假设为有效疟疾传播的重要因素，但这从未得到正式测试。在布基纳法索自然感染的配子体携带者中，我们通过直接皮肤喂养和膜喂养来评估对蚊子的传染性。我们对采食静脉血或直接采食皮肤的蚊子的手指刺血和静脉血、皮肤活检样本和雄性、雌性配子体和无性寄生虫进行了定量分析。用共聚焦显微镜观察皮肤组织中的配子体。虽然直接取食皮肤比取食静脉血更容易感染蚊子（优势比为2.01,95%可信区间为1.21-3.33,P = .007），但皮下皮肤血管中的配子细胞浓度并不高于其他血室；皮肤组织中仅见稀疏配子体。我们的数据强烈表明，恶性疟原虫配子体没有明显的皮肤隔离。因此，外周血中的配子体密度为预测向蚊子传播的可能性提供了信息，并可用于定位和监测消除疟疾的行动。恶性疟原虫配子细胞皮肤隔离长期以来一直是假设，但从未正式测试。我们没有观察到在自然感染的配子细胞携带者的皮肤组织或以其皮肤为食的蚊子的血中有更高的配子细胞密度的证据。
Plasmodium falciparum transmission depends on mature gametocytes that can be ingested by mosquitoes taking a blood meal on human skin. Although gametocyte skin sequestration has long been hypothesized as important contributor to efficient malaria transmission, this has never been formally tested. In naturally infected gametocyte carriers from Burkina Faso, we assessed infectivity to mosquitoes by direct skin feeding and membrane feeding. We directly quantified male and female gametocytes and asexual parasites in finger-prick and venous blood samples, skin biopsy samples, and in of mosquitoes that fed on venous blood or directly on skin. Gametocytes were visualized in skin tissue with confocal microscopy. Although more mosquitoes became infected when feeding directly on skin then when feeding on venous blood (odds ratio, 2.01; 95% confidence interval, 1.21–3.33; P = .007), concentrations of gametocytes were not higher in the subdermal skin vasculature than in other blood compartments; only sparse gametocytes were observed in skin tissue. Our data strongly suggest that there is no significant skin sequestration of P. falciparum gametocytes. Gametocyte densities in peripheral blood are thus informative for predicting onward transmission potential to mosquitoes and can be used to target and monitor malaria elimination initiatives. Plasmodium falciparum gametocyte skin sequestration has long been hypothesized but never formally tested. We observed no evidence for higher gametocyte densities in skin tissue of naturally infected gametocyte carriers or blood meals of mosquitoes feeding on their skin.
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DOI：
10.5281/zenodo.10926272
发表时间：
2013-01-01
期刊：
MalariaWorld journal
影响因子：
--
作者：
Ouedraogo, Andre Lin;Guelbeogo, Wamdaogo M;Bousema, Teun
通讯作者：
Bousema, Teun
影响因子：
17.1
作者：
Joice R;Nilsson SK;Montgomery J;Dankwa S;Egan E;Morahan B;Seydel KB;Bertuccini L;Alano P;Williamson KC;Duraisingh MT;Taylor TE;Milner DA;Marti M
通讯作者：
Marti M
影响因子：
64.5
作者：
BARUCH, DI;PASLOSKE, BL;HOWARD, RJ
通讯作者：
HOWARD, RJ
影响因子：
20.3
作者：
Naissant, Bernina;Dupuy, Florian;Lavazec, Catherine
通讯作者：
Lavazec, Catherine
影响因子：
56.3
作者：
Dicko, Alassane;Brown, Joelle M.;Gosling, Roly
通讯作者：
Gosling, Roly