Mechanistic studies of the biosynthesis of 2-thiosugar: evidence for the formation of an enzyme-bound 2-ketohexose intermediate in BexX-catalyzed reaction.
Mechanistic studies of the biosynthesis of 2-thiosugar: evidence for the formation of an enzyme-bound 2-ketohexose intermediate in BexX-catalyzed reaction.
复制标题
DOI：
10.1021/ja108061c
复制
发表时间：
2010-11-10
影响因子：
15
通讯作者：
Liu HW
中科院分区：
文献类型：
--
作者：
Sasaki E;Liu HW
本文首次报道了安古霉素类抗生素BE-7585 A产生2-巯基糖的机理。通过捕获共价键合的酶-底物中间体，D-葡萄糖6-磷酸被鉴定为推定的硫代糖生物合成蛋白BexX的底物。通过诱变研究和LC-MS/MS分析确定K110残基的位点特异性修饰。一个关键的中间体携带酮功能被证实存在于酶-底物复合物。这些结果表明，硫插入机制在2-巯基糖的生物合成共享的硫胺素生物合成的相似性。
The first mechanistic insight into the 2-thiosugar production in an angucycline-type antibiotic, BE-7585A is reported. D-Glucose 6-phosphate was identified as the substrate for the putative thiosugar biosynthetic protein, BexX, by trapping the covalently bonded enzyme-substrate intermediate. The site-specific modification at K110 residue was determined by mutagenesis studies and LC-MS/MS analysis. A key intermediate carrying a keto functionality was confirmed to exist in the enzyme-substrate complex. These results suggest that the sulfur insertion mechanism in 2-thiosugar biosynthesis shares similarities with that for thiamin biosynthesis.
登录
查看更多内容
影响因子：
16.6
作者：
Jurgenson CT;Begley TP;Ealick SE
通讯作者：
Ealick SE
影响因子：
15
作者：
Sasaki, Eita;Ogasawara, Yasushi;Liu, Hung-wen
通讯作者：
Liu, Hung-wen
影响因子：
4
作者：
Melancon, Charles E., III;Thibodeaux, Christopher J.;Liu, Hung-wen
通讯作者：
Liu, Hung-wen
影响因子：
46.9
作者：
Madduri, K;Kennedy, J;Hutchinson, CR
通讯作者：
Hutchinson, CR
影响因子：
4.8
作者：
Mouilleron, S;Badet-Denisot, MA;Golinelli-Pimpaneau, B
通讯作者：
Golinelli-Pimpaneau, B