Palmitoylated Ras proteins traffic through recycling endosomes to the plasma membrane during exocytosis.
Palmitoylated Ras proteins traffic through recycling endosomes to the plasma membrane during exocytosis.
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DOI：
10.1083/jcb.200911143
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发表时间：
2010-10-04
期刊：
影响因子：
--
通讯作者：
Taguchi T
中科院分区：
文献类型：
--
作者：
Misaki R;Morimatsu M;Uemura T;Waguri S;Miyoshi E;Taniguchi N;Matsuda M;Taguchi T
棕榈酰化将Ras蛋白导向正确的细胞内细胞器进行运输和活性。Ras蛋白调节细胞生长、死亡和分化，并且已经确定这种功能多样性是通过它们不同的亚细胞定位来实现的。棕榈酰化的H-和N-Ras被认为定位于核周高尔基体和质膜（PM）。然而，值得注意的是，再循环内体（RE）也定位于核周区域，其通常与高尔基体难以区分。在这项研究中，我们发现，活性棕榈酰化Ras蛋白主要定位在RE细胞内和RE作为一个小站沿着后高尔基体的胞外途径PM。H-Ras需要两个棕榈酰基用于RE靶向。棕榈酰基团中的一个或两个的缺乏导致突变蛋白质错误定位于内质网、高尔基体或PM。因此，我们证明棕榈酰化指导Ras蛋白正确的细胞内细胞器的运输和活动。
Palmitoylation directs Ras proteins to the correct intracellular organelles for trafficking and activity. Ras proteins regulate cell growth, death, and differentiation, and it is well established that this functional versatility is accomplished through their different subcellular localizations. Palmitoylated H- and N-Ras are believed to localize at the perinuclear Golgi and plasma membrane (PM). Notably, however, recycling endosomes (REs) also localize to a perinuclear region, which is often indistinguishable from the Golgi. In this study, we show that active palmitoylated Ras proteins mainly localize intracellularly at REs and that REs act as a way station along the post-Golgi exocytic pathway to the PM. H-Ras requires two palmitoyl groups for RE targeting. The lack of either or both palmitoyl groups leads to the mislocalization of the mutant proteins to the endoplasmic reticulum, Golgi apparatus, or the PM. Therefore, we demonstrate that palmitoylation directs Ras proteins to the correct intracellular organelles for trafficking and activity.
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