Cell-type-specific circuit connectivity of hippocampal CA1 revealed through Cre-dependent rabies tracing.
Cell-type-specific circuit connectivity of hippocampal CA1 revealed through Cre-dependent rabies tracing.
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DOI：
10.1016/j.celrep.2014.02.030
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发表时间：
2014-04-10
期刊：
影响因子：
8.8
通讯作者：
Xu X
中科院分区：
文献类型：
--
作者：
Sun Y;Nguyen AQ;Nguyen JP;Le L;Saur D;Choi J;Callaway EM;Xu X
我们应用了一种新的cre依赖的、基于狂犬病的转基因示踪系统来绘制小鼠海马中CA1兴奋性和抑制性神经元类型的直接突触连接。我们发现兴奋性和抑制性CA1神经元的共同输入来自CA3、CA2、内嗅皮层和内侧隔（MS），出乎意料的是，也来自耻骨下。兴奋性CA1神经元接受来自胆碱能和gaba能MS神经元的输入，而抑制性CA1神经元接受来自gaba能MS神经元的绝大部分输入；这两种细胞类型也从谷氨酸能MS神经元接收较弱的输入。对CA1的PV+中间神经元与SOM+中间神经元的输入比较显示，来自丘下的输入强度相似，但PV+中间神经元接受的输入比来自CA3、内嗅皮层和ms的SOM+神经元强得多，从CA3到特定CA1细胞类型的差异输入也通过激光扫描光刺激和全细胞记录得到功能证明。
We applied a new Cre-dependent, genetically modified rabies-based tracing system to map direct synaptic connections to CA1 excitatory and inhibitory neuron types in mouse hippocampus. We found common inputs to excitatory and inhibitory CA1 neurons from CA3, CA2, entorhinal cortex and the medial septum (MS), and unexpectedly also from the subiculum. Excitatory CA1 neurons receive inputs from both cholinergic and GABAergic MS neurons while inhibitory CA1 neurons receive a great majority of input from GABAergic MS neurons; both cell types also receive weaker input from glutamatergic MS neurons. Comparisons of inputs to CA1 PV+ interneurons versus SOM+ interneurons showed similar strengths of input from the subiculum, but PV+ interneurons receive much stronger input than SOM+ neurons from CA3, entorhinal cortex and MS. Differential input from CA3 to specific CA1 cell types was also demonstrated functionally using laser scanning photostimulation and whole cell recordings.
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影响因子：
25
作者：
Cruikshank, Scott J.;Lewis, Timothy J.;Connors, Barry W.
通讯作者：
Connors, Barry W.
影响因子：
56.9
作者：
Nakashiba, Toshiaki;Young, Jennie Z.;Tonegawa, Susumu
通讯作者：
Tonegawa, Susumu
影响因子：
5.3
作者：
Huh, Carey Y. L.;Goutagny, Romain;Williams, Sylvain
通讯作者：
Williams, Sylvain
DOI：
10.1073/pnas.0308577100
发表时间：
2004-02-24
影响因子：
11.1
作者：
Brivanlou, IH;Dantzker, JLM;Callaway, EM
通讯作者：
Callaway, EM
影响因子：
56.9
作者：
Brun, VH;Otnæss, MK;Moser, EI
通讯作者：
Moser, EI