Determination of fentanyl in human breath by solid-phase microextraction and gas chromatography–mass spectrometry
Determination of fentanyl in human breath by solid-phase microextraction and gas chromatography–mass spectrometry
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固相微萃取气相色谱-质谱法测定人呼吸中的芬太尼
DOI：
10.1016/j.microc.2008.09.002
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发表时间：
2009-03
影响因子：
4.8
通讯作者：
中科院分区：
文献类型：
--
作者：
芬太尼是一种静脉麻醉性镇痛剂，广泛应用于临床麻醉。作为一种潜在的污染，在心胸外科手术室的空气和患者的呼气回路中都检测到了它。然而，患者呼气回路中的芬太尼是否被患者呼出尚不清楚。本研究从静脉注射芬太尼患者的麻醉机呼气回路中采集呼吸样本，建立了固相微萃取(SPME)-气相色谱-质谱仪(GC-MS)联用检测呼吸样本中芬太尼的方法。考察了影响分析物在纤维上的吸附(提取时间、温度)和解吸(解吸时间)的参数，并为方法开发进行了验证。该方法具有操作简便、成本低廉、灵敏度高、重复性好等特点。线性范围为0.05~0.8 ng/mL。方法的检出限为0.01 ng/m L，日内精密度小于12.13%(n=5)。本研究涉及6例患者，结果显示静脉注射芬太尼的患者呼吸中存在芬太尼，呼吸中芬太尼的浓度范围为6.00~20.89 pg/mL。总之，接受芬太尼静脉注射的患者可以呼出芬太尼。
Fentanyl, a kind of intravenous narcotic analgesic, is widely used in clinical anesthesia. As a potential pollution, it was detected in both the air of the cardiothoracic operating room and patients' expiratory circuit. However, whether the fentanyl in patients' expiratory circuit is exhaled by patients is unknown. In this study, breath samples were taken from the expiratory circuits of anesthetic machine linked to the patients who received intravenous fentanyl, a solid-phase microextraction (SPME) coupled with gas chromatography–mass spectrometry (GC–MS) method was developed to detect and quantify fentanyl in breath samples. The parameters influencing adsorption (extraction time, temperature,) and desorption (desorption time) of the analyte on the fiber were investigated and validated for method development. The developed method was proved to be simple, easy, and inexpensive and offer high sensitivity and reproducibility. Linear range was obtained from 0.05 ng/mL to 0.8 ng/mL. The limit of detection was 0.01 ng/mL while an interday precision of less than 12.13% (n=5) could be achieved. Six patients were involved in this study; results showed presence of fentanyl in the breath of patients who received intravenous fentanyl, and fentanyl concentrations in breath varied from 6.00 to 20.89 pg/mL. In conclusion, fentanyl can be exhaled by patients who received intravenous fentanyl.
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影响因子：
5.7
作者：
J. Silverstein;M. Rieders;M. McMullin;S. Schulman;K. Zahl
通讯作者：
J. Silverstein;M. Rieders;M. McMullin;S. Schulman;K. Zahl
影响因子：
4.8
作者：
Li Yuansheng;Wu Yutian;Z. Jing;Zhai Zhenxing;Qi Zhanxi;G. Shen;Kong Qinghong;W. Xinhua
通讯作者：
Li Yuansheng;Wu Yutian;Z. Jing;Zhai Zhenxing;Qi Zhanxi;G. Shen;Kong Qinghong;W. Xinhua
影响因子：
4.7
作者：
McAuliffe, PF;Gold, MS;Cendán, JC
通讯作者：
Cendán, JC
影响因子：
2.5
作者：
C. Paradis;C. Dufresne;M. Bolon;R. Boulieu
通讯作者：
C. Paradis;C. Dufresne;M. Bolon;R. Boulieu
DOI：
10.1016/j.chroma.2004.02.074
发表时间：
2004-05
期刊：
Journal of chromatography. A
影响因子：
--
作者：
N. F. J. Van Nimmen;H. Veulemans
通讯作者：
N. F. J. Van Nimmen;H. Veulemans