The clam 3' UTR masking element-binding protein p82 is a member of the CPEB family.
The clam 3' UTR masking element-binding protein p82 is a member of the CPEB family.
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蛤 3 UTR 掩蔽元件结合蛋白 p82 是 CPEB 家族的成员。
DOI：
10.1017/s1355838299981219
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发表时间：
1999
期刊：
影响因子：
4.5
通讯作者：
N. Standart
中科院分区：
文献类型：
--
作者：
J. Walker;N. Minshall;L. Hake;J. Richter;N. Standart
在早期发育期间，基因表达主要在翻译水平上受到控制。北蛤 Spisula Solidissima 的卵母细胞含有大量的母体 mRNA 储备，这些 mRNA 在翻译上处于休眠或隐藏状态，直到减数分裂成熟。受精对卵母细胞的激活导致丰富的细胞周期蛋白和核糖核苷酸还原酶 mRNA 在细胞质多聚腺苷酸化时翻译激活。体外揭掩测定已将这些 mRNA 的 3' UTR 大约中心位置的富含 U 的区域定义为翻译掩蔽元件。 82 kDa 的蛤卵母细胞蛋白 p82 可选择性结合掩蔽元件，已被提议充当翻译阻遏蛋白。重要的是，体外 mRNA 特异性揭开是在不存在 Poly(A) 延伸的情况下发生的。在这里，我们发现蛤 p82 与爪蟾 CPEB 相关，爪蟾 CPEB 是一种 RNA 结合蛋白，可与母体 mRNA 的富含 U 的细胞质聚腺苷酸化元件 (CPE) 相互作用，并促进其聚腺苷酸化。克隆的蛤 p82/CPEB 显示出与爪蟾 CPEB 以及来自小鼠、金鱼、果蝇和秀丽隐杆线虫的相关多肽的广泛同源性，特别是在它们的 RNA 结合 C 末端半部分。两个短的 N 端序列岛（功能未知）是脊椎动物 CPEB 和蛤 p82 所共有的。在减数分裂成熟的蛤卵母细胞中受精后，p82 在第一次细胞分裂过程中降解之前，直接或间接地被 cdc2 激酶快速磷酸化。磷酸化先于母体 mRNA 的翻译激活，并且根据抑制剂研究，磷酸化可能是母体 mRNA 翻译激活所必需的。这些数据表明 clam p82 可能是 Xenopus CPEB 的功能同源物。
During early development gene expression is controlled principally at the translational level. Oocytes of the surf clam Spisula solidissima contain large stockpiles of maternal mRNAs that are translationally dormant or masked until meiotic maturation. Activation of the oocyte by fertilization leads to translational activation of the abundant cyclin and ribonucleotide reductase mRNAs at a time when they undergo cytoplasmic polyadenylation. In vitro unmasking assays have defined U-rich regions located approximately centrally in the 3' UTRs of these mRNAs as translational masking elements. A clam oocyte protein of 82 kDa, p82, which selectively binds the masking elements, has been proposed to act as a translational repressor. Importantly, mRNA-specific unmasking in vitro occurs in the absence of poly(A) extension. Here we show that clam p82 is related to Xenopus CPEB, an RNA-binding protein that interacts with the U-rich cytoplasmic polyadenylation elements (CPEs) of maternal mRNAs and promotes their polyadenylation. Cloned clam p82/CPEB shows extensive homology to Xenopus CPEB and related polypeptides from mouse, goldfish, Drosophila and Caenorhabditis elegans, particularly in their RNA-binding C-terminal halves. Two short N-terminal islands of sequence, of unknown function, are common to vertebrate CPEBs and clam p82. p82 undergoes rapid phosphorylation either directly or indirectly by cdc2 kinase after fertilization in meiotically maturing clam oocytes, prior to its degradation during the first cell cleavage. Phosphorylation precedes and, according to inhibitor studies, may be required for translational activation of maternal mRNA. These data suggest that clam p82 may be a functional homolog of Xenopus CPEB.
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影响因子：
10.5
作者：
J. Paris;K. Swenson;H. Piwnica-Worms;J. Richter
通讯作者：
J. Paris;K. Swenson;H. Piwnica-Worms;J. Richter
控制细胞质聚腺苷酸化的序列元件的进化保守性。
DOI：
10.1073/pnas.93.17.9027
发表时间：
1996
影响因子：
11.1
作者：
Verrotti,AC;Thompson,SR;Wreden,C;Strickland,S;Wickens,M
通讯作者：
Wickens,M
揭示蛤卵母细胞中的 mRNA：受精时 3UTR 掩蔽元件结合蛋白磷酸化的作用。
DOI：
10.1006/dbio.1996.0024
发表时间：
1996
期刊：
Developmental biology.
影响因子：
--
作者：
Walker,J;Dale,M;Standart,N
通讯作者：
Standart,N
影响因子：
10.5
作者：
CHRISTERSON, LB;MCKEARIN, DM
通讯作者：
MCKEARIN, DM