Early molecular events associated with nitrogen deficiency in rice seedling roots.
Early molecular events associated with nitrogen deficiency in rice seedling roots.
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DOI：
10.1038/s41598-018-30632-1
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发表时间：
2018-08-15
影响因子：
4.6
通讯作者：
Hsieh MH
中科院分区：
文献类型：
--
作者：
Hsieh PH;Kan CC;Wu HY;Yang HC;Hsieh MH
氮素缺乏症是水稻最常见的问题之一。氮缺乏的症状已被充分记录，但水稻中潜在的分子机制在很大程度上是未知的。在这里，我们研究了与N饥饿（- N, 1 h）相关的早期分子事件，重点研究了水稻根系中- N调控基因的氨基酸分析和鉴定。有趣的是，谷氨酰胺水平在−N处理后15分钟内迅速下降，表明部分缺氮信号可能是由谷氨酰胺介导的。转录组分析显示，参与代谢、植物激素信号转导（如脱落酸、生长素和茉莉酸）、转运蛋白活性和氧化应激反应的基因受到−N的快速调控。一些负氮调控基因编码转录因子、蛋白激酶和蛋白磷酸酶，这些基因可能参与水稻根系早期负氮响应的调控。以前，我们使用类似的方法来鉴定谷氨酰胺，谷氨酸和硝酸铵反应基因。将不同形态氮素诱导的基因与本研究发现的- N调控基因进行比较，为进一步剖析水稻氮素信号通路提供了潜在的N调控基因目录。
Nitrogen (N) deficiency is one of the most common problems in rice. The symptoms of N deficiency are well documented, but the underlying molecular mechanisms are largely unknown in rice. Here, we studied the early molecular events associated with N starvation (−N, 1 h), focusing on amino acid analysis and identification of −N-regulated genes in rice roots. Interestingly, levels of glutamine rapidly decreased within 15 min of −N treatment, indicating that part of the N-deficient signals could be mediated by glutamine. Transcriptome analysis revealed that genes involved in metabolism, plant hormone signal transduction (e.g. abscisic acid, auxin, and jasmonate), transporter activity, and oxidative stress responses were rapidly regulated by −N. Some of the −N-regulated genes encode transcription factors, protein kinases and protein phosphatases, which may be involved in the regulation of early −N responses in rice roots. Previously, we used similar approaches to identify glutamine-, glutamate-, and ammonium nitrate-responsive genes. Comparisons of the genes induced by different forms of N with the −N-regulated genes identified here have provided a catalog of potential N regulatory genes for further dissection of the N signaling pathwys in rice.
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影响因子：
7.4
作者：
Giehl, Ricardo F. H.;von Wiren, Nicolaus
通讯作者：
von Wiren, Nicolaus
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DOI：
10.1073/pnas.0813417106
发表时间：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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