Laminar expression of ephrin-A2 in primary somatosensory cortex of postnatal rats.
Laminar expression of ephrin-A2 in primary somatosensory cortex of postnatal rats.
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DOI：
10.1002/ar.21485
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发表时间：
2012-01
影响因子：
2
通讯作者：
Mooney, Richard D.
中科院分区：
文献类型：
--
作者：
Kenmuir, Cynthia L.;Chiaia, Nicolas L.;Lane, Richard D.;Mooney, Richard D.
已知几种Eph受体，主要是EphA4和EphA7，及其相应的配体影响新皮层发育，包括初级体感皮层（SI）内丘脑皮层轴突的地形分类。本研究调查出生后的表达的配体，可以结合到这些受体，肝配蛋白A2。定量方法显示，ephrin-A2 mRNA在SI中的表达在出生后第4天达到最高水平（P），此后下降到P18的背景。Ephrin-A2 mRNA的表达通过原位杂交定性评估揭示了一个类似的时间过程和本地化的表达模式主要在两个广泛的板层在SI，包括颗粒上和颗粒下层，并与额外的表达在subplate。使用肝配蛋白-A2蛋白的免疫组织化学更详细地研究了这种表达模式。免疫反应性一般表现出相同的层状分布与原位杂交，除了它持续时间更长，持续约P14。在皮质板中的表达是低的或不存在的假定层IV，它仍然是这样的皮质分层进展。免疫组织化学双标记共聚焦显微镜显示，皮质神经元的ephrin-A2蛋白表达的主要元素。这些研究结果是一致的可能参与ephrin-A2，在音乐会与一个或多个Eph受体，在影响乔木发展的丘脑皮层轴突在皮层第四层边界。
Several Eph receptors, prominently EphA4 and EphA7, and their corresponding ligands are known to influence neocortical development, including topographic sorting of thalamocortical axons within primary somatosensory cortex (SI). The present study investigated postnatal expression of a ligand that can bind to these receptors, ephrin-A2. Quantitative methods revealed that expression of ephrin-A2 mRNA in SI reached maximum levels on postnatal day (P) 4 and dropped thereafter to background by P18. Ephrin-A2 mRNA expression assessed by in situ hybridization qualitatively revealed a similar time course and localized the expression pattern primarily in two broad laminae in SI, comprising the supragranular and infragranular layers, and with additional expression in the subplate. This expression pattern was investigated in greater detail using immunohistochemistry for ephrin-A2 protein. Immunoreactivity generally showed the same laminar distribution as seen with in situ hybridization, except that it persisted longer, lasting to approximately P14. Expression in the cortical plate was low or absent within presumptive layer IV, and it remained so as cortical lamination progressed. Double-labeling immunohistochemistry with confocal microscopy revealed that cortical neurons were the principal elements expressing ephrin-A2 protein. These findings are consistent with possible involvement of ephrin-A2, in concert with one or more Eph receptors, in influencing arbor development of thalamocortical axons at cortical layer IV boundaries.
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