Involvement of type VI secretion system in secretion of iron chelator pyoverdine in Pseudomonas taiwanensis.
Involvement of type VI secretion system in secretion of iron chelator pyoverdine in Pseudomonas taiwanensis.
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DOI：
10.1038/srep32950
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发表时间：
2016-09-08
影响因子：
4.6
通讯作者：
Shih MC
中科院分区：
文献类型：
--
作者：
Chen WJ;Kuo TY;Hsieh FC;Chen PY;Wang CS;Shih YL;Lai YM;Liu JR;Yang YL;Shih MC
水稻白叶枯病菌。水稻白叶枯病（Xoo）是世界上危害性最大的水稻病害之一。因此，除了培育抗病水稻品种外，还需要开发有效的Xoo生物防治剂。在这里，我们报告，土壤细菌假单胞菌台湾显示出强烈的拮抗活性对Xoo。使用基质辅助激光解吸/电离成像质谱，我们鉴定了台湾假单胞菌分泌的铁螯合剂绿脓菌荧光素，其可以抑制Xoo的生长。通过台湾假单胞菌的Tn 5诱变，我们显示编码VI型分泌系统（T6 SS）的组分的基因中的突变以及绿脓菌荧光素的生物合成和成熟导致针对Xoo的毒性降低。我们的结果表明，T6 SS参与内源性绿脓菌荧光素的分泌。T6 SS组分基因中的突变影响了在绿脓菌荧光素前体通过内膜转运蛋白PvdE转移到周质后，成熟绿脓菌荧光素从周质空间分泌到细胞外培养基中。此外，我们还表明，其他出口系统，即，PvdRT-OpmQ和MexAB-OprM外排系统（先前已暗示涉及）和II型分泌系统（T2 SS）不涉及绿脓菌荧光素分泌。
Rice bacterial blight caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the most destructive rice diseases worldwide. Therefore, in addition to breeding disease-resistant rice cultivars, it is desirable to develop effective biocontrol agents against Xoo. Here, we report that a soil bacterium Pseudomonas taiwanensis displayed strong antagonistic activity against Xoo. Using matrix-assisted laser desorption/ionization imaging mass spectrometry, we identified an iron chelator, pyoverdine, secreted by P. taiwanensis that could inhibit the growth of Xoo. Through Tn5 mutagenesis of P. taiwanensis, we showed that mutations in genes that encode components of the type VI secretion system (T6SS) as well as biosynthesis and maturation of pyoverdine resulted in reduced toxicity against Xoo. Our results indicated that T6SS is involved in the secretion of endogenous pyoverdine. Mutations in T6SS component genes affected the secretion of mature pyoverdine from the periplasmic space into the extracellular medium after pyoverdine precursor is transferred to the periplasm by the inner membrane transporter PvdE. In addition, we also showed that other export systems, i.e., the PvdRT-OpmQ and MexAB-OprM efflux systems (for which there have been previous suggestions of involvement) and the type II secretion system (T2SS), are not involved in pyoverdine secretion.
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影响因子：
6.7
作者：
Cianfanelli FR;Alcoforado Diniz J;Guo M;De Cesare V;Trost M;Coulthurst SJ
通讯作者：
Coulthurst SJ
影响因子：
3.4
作者：
Imperi, Francesco;Ciccosanti, Fabiola;Fimia, Gian Maria
通讯作者：
Fimia, Gian Maria
影响因子：
3.5
作者：
McMorran, BJ;Merriman, ME;Lamont, IL
通讯作者：
Lamont, IL
影响因子：
3.2
作者：
HUANG, HC;HE, SY;COLLMER, A
通讯作者：
COLLMER, A
影响因子：
3.5
作者：
Hannauer, Melissa;Schaefer, Mathias;Schalk, Isabelle J.
通讯作者：
Schalk, Isabelle J.