In vitro guidance of retinal axons by a tectal lamina-specific glycoprotein Nel.
In vitro guidance of retinal axons by a tectal lamina-specific glycoprotein Nel.
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DOI：
10.1016/j.mcn.2009.02.006
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发表时间：
2009-06
影响因子：
3.5
通讯作者：
Nakamoto, Masaru
中科院分区：
文献类型：
--
作者：
Jiang, Yulan;Obama, Hiroya;Kuan, Soh Leh;Nakamura, Ritsuko;Nakamoto, Chizu;Ouyang, Zhufeng;Nakamoto, Masaru
Nel是一种糖蛋白，含有5个索蛋白样结构域和6个表皮生长因子样结构域，在神经系统中强烈表达。在这项研究中，我们研究了表达模式和体外功能的Nel在鸡视网膜顶盖系统。我们已经发现，在发育中的顶盖中，Nel的表达定位于视网膜轴突通常不进入的特定板层中，包括视网膜受体板层和非视网膜受体板层之间的边界。在体内和体外的视网膜轴突上检测到Nel结合活性，表明视网膜轴突表达Nel的受体。在体外，Nel抑制视网膜轴突生长并诱导生长锥塌陷和轴突回缩。这些结果表明，Nel作为视网膜轴突的抑制性引导线索，并建议其在建立层特异性的视网膜顶盖投射的作用。
Nel is a glycoprotein containing five chordin-like and six epidermal growth factor-like domains and is strongly expressed in the nervous system. In this study, we have examined expression patterns and in vitro functions of Nel in the chicken retinotectal system. We have found that in the developing tectum, expression of Nel is localized in specific laminae that retinal axons normally do not enter, including the border between the retino-recipient and non-retinorecipient laminae. Nel-binding activity is detected on retinal axons both in vivo and in vitro, suggesting that retinal axons express a receptor for Nel. In vitro, Nel inhibits retinal axon outgrowth and induces growth cone collapse and axon retraction. These results indicate that Nel acts as an inhibitory guidance cue for retinal axons, and suggest its roles in the establishment of the lamina-specificity in the retinotectal projection.
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