Obscurin depletion impairs organization of skeletal muscle in developing zebrafish embryos.
Obscurin depletion impairs organization of skeletal muscle in developing zebrafish embryos.
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DOI：
10.1155/2011/479135
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发表时间：
2011
影响因子：
--
通讯作者：
Russell MW
中科院分区：
文献类型：
--
作者：
Raeker MÖ;Russell MW
在发育过程中，骨骼肌成肌细胞分化为具有相对于周围细胞和组织精确定向的成熟收缩结构的肌细胞和骨骼肌管。这种高度有序结构的建立需要分化中的肌细胞和周围的细胞外基质之间的相互作用，以形成从骨骼肌到骨骼的正确定位和良好组织的附着。以发育中的斑马鱼胚胎为模型，我们研究了新的肌原纤维组装和细胞-细胞外基质相互作用中所涉及的膜结构域的组织之间的关系。我们确定，obscurin，一个巨大的肌肉蛋白质，耗尽，导致不规则的细胞形态和骨骼肌发育过程中的细胞外基质组织紊乱。由此产生的肌细胞组织损伤与肌细胞内部结构的干扰有关，这表明obscurin参与组织肌细胞的内部结构并将这些结构线索转化为周围细胞和组织。
During development, skeletal myoblasts differentiate into myocytes and skeletal myotubes with mature contractile structures that are precisely oriented with respect to surrounding cells and tissues. Establishment of this highly ordered structure requires reciprocal interactions between the differentiating myocytes and the surrounding extracellular matrix to form correctly positioned and well-organized attachments from the skeletal muscle to the bony skeleton. Using the developing zebrafish embryo as a model, we examined the relationship between new myofibril assembly and the organization of the membrane domains involved in cell-extracellular matrix interactions. We determined that depletion of obscurin, a giant muscle protein, resulted in irregular cell morphology and disturbed extracellular matrix organization during skeletal muscle development. The resulting impairment of myocyte organization was associated with disturbance of the internal architecture of the myocyte suggesting that obscurin participates in organizing the internal structure of the myocyte and translating those structural cues to surrounding cells and tissues.
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DOI：
10.1083/jcb.132.5.835
发表时间：
1996-03
期刊：
The Journal of cell biology
影响因子：
--
作者：
Benian GM;Tinley TL;Tang X;Borodovsky M
通讯作者：
Borodovsky M
影响因子：
2.5
作者：
Kim, H. Rosemary;Ingham, Philip W.
通讯作者：
Ingham, Philip W.
影响因子：
10.5
作者：
Durbin, L;Brennan, C;Holder, N
通讯作者：
Holder, N
影响因子：
4.6
作者：
Juelich, Doerthe;Mould, A. Paul;Holley, Scott A.
通讯作者：
Holley, Scott A.
影响因子：
2.5
作者：
Raeker, Maide O.;Su, Fengyun;Russell, Mark W.
通讯作者：
Russell, Mark W.