Assessment of metabolic patterns and new antitumoral treatment in osteosarcoma xenograft models by [(18)F]FDG and sodium [(18)F]fluoride PET.
Assessment of metabolic patterns and new antitumoral treatment in osteosarcoma xenograft models by [(18)F]FDG and sodium [(18)F]fluoride PET.
复制标题
[（18）F] FDG和钠[（18）F]氟化物PET评估骨肉瘤异种移植模型中代谢模式和新的抗肿瘤治疗。
DOI：
10.1186/s12885-018-5122-y
复制
发表时间：
2018-11-29
期刊：
影响因子：
3.8
通讯作者：
Peñuelas I
中科院分区：
文献类型：
--
作者：
Collantes M;Martínez-Vélez N;Zalacain M;Marrodán L;Ecay M;García-Velloso MJ;Alonso MM;Patiño-García A;Peñuelas I
骨肉瘤是儿童和年轻人最常见的恶性骨肿瘤，可产生异常的类骨质。本研究的目的是评估2-脱氧-2-[18 F-]氟-D-葡萄糖（[18 F] FDG）和[18 F]氟化钠（Na [18 F] F）PET扫描在骨肉瘤原位小鼠模型中的效用，以描述肿瘤的代谢模式，检测和诊断肿瘤，并评估基于溶瘤腺病毒的新治疗的疗效。通过注射143 B和531 MII细胞系建立原位骨肉瘤小鼠模型。注射后30天（143 B）和90天（531 MII）进行[18 F]FDG和Na [18 F] F PET扫描。通过[18F] FDG PET研究在531 MII模型中评价两种剂量（107和108 pfu）的溶瘤腺病毒VCN-01的抗肿瘤作用。[18F]FDG摄取通过SUVmax和总病变糖酵解（TLG）指数进行定量。对于Na [18 F] F，计算肿瘤SUVmax/髋关节SUVmax的比值。PET结果通过组织病理学技术证实。两种原位模型的肿瘤代谢模式不同。所有肿瘤均显示[18F] FDG摄取，敏感性和特异性均为100%。143 B模型的[18F] FDG摄取显著更高（p < 0.001）。在两种模型中，Na [18 F] F的灵敏度约为70%，特异性为100%。531 MII肿瘤显示出不均匀的Na [18 F] F摄取，显著高于143 B肿瘤（p < 0.01）。重要的是，在组织病理学分析中，[18 F] FDG和Na [18 F] F摄取分别对应于高细胞或类骨质丰富的肿瘤。[18F]FDG数据证实，即使使用107 pfu剂量，531 MII肿瘤的溶瘤治疗也产生了生长的显著降低。PET研究表明，不同的骨肉瘤异种移植模型发展的肿瘤具有不同的代谢模式，可以通过多示踪剂PET研究来描述。由于并非所有肿瘤都产生丰富的类骨质，[18 F] FDG表现出更好的肿瘤检测灵敏度，并能够定量监测体内对溶瘤腺病毒VCN-01的反应。本文的在线版本（10.1186/s12885-018-5122-y）包含补充材料，可供授权用户使用。
Osteosarcoma is the most common malignant bone tumor in children and young adults that produces aberrant osteoid. The aim of this study was to assess the utility of 2-deoxy-2-[18F-] fluoro-D-glucose ([18F] FDG) and sodium [18F] Fluoride (Na [18F] F) PET scans in orthotopic murine models of osteosarcoma to describe the metabolic pattern of the tumors, to detect and diagnose tumors and to evaluate the efficacy of a new treatment based in oncolytic adenoviruses. Orthotopic osteosarcoma murine models were created by the injection of 143B and 531MII cell lines. [18F]FDG and Na [18F] F PET scans were performed 30 days (143B) and 90 days (531MII) post-injection. The antitumor effect of two doses (107 and 108 pfu) of the oncolytic adenovirus VCN-01 was evaluated in 531 MII model by [18F] FDG PET studies. [18F] FDG uptake was quantified by SUVmax and Total Lesion Glycolysis (TLG) indexes. For Na [18F] F, the ratio tumor SUVmax/hip SUVmax was calculated. PET findings were confirmed by histopathological techniques. The metabolic pattern of tumors was different between both orthotopic models. All tumors showed [18F] FDG uptake, with a sensitivity and specificity of 100%. The [18F] FDG uptake was significantly higher for the 143B model (p < 0.001). Sensitivity for Na [18F] F was around 70% in both models, with a specificity of 100%. 531MII tumors showed a heterogeneous Na [18F] F uptake, significantly higher than 143B tumors (p < 0.01). Importantly, [18F] FDG and Na [18F] F uptake corresponded to highly cellular or osteoid-rich tumors in the histopathological analysis, respectively. [18F] FDG data confirmed that the oncolytic treatment of 531MII tumors produced a significant reduction in growth even with the 107 pfu dose. PET studies demonstrated that the different osteosarcoma xenograft models developed tumors with diverse metabolic patterns that can be described by multitracer PET studies. Since not all tumors produced abundant osteoid, [18F] FDG demonstrated a better sensitivity for tumor detection and was able to quantitatively monitor in vivo response to the oncolytic adenovirus VCN-01. The online version of this article (10.1186/s12885-018-5122-y) contains supplementary material, which is available to authorized users.
登录
查看更多内容
影响因子：
64.8
作者：
Greaves, Mel;Maley, Carlo C.
通讯作者：
Maley, Carlo C.
影响因子：
2.1
作者：
Byun, Byung Hyun;Kong, Chang-Bae;Lim, Sang Moo
通讯作者：
Lim, Sang Moo
DOI：
10.1007/s00259-011-1934-6
发表时间：
2012-01-01
影响因子：
9.1
作者：
Liao, Shengri;Penney, Bill C.;Pu, Yonglin
通讯作者：
Pu, Yonglin
影响因子：
3.5
作者：
Klein, MJ;Siegal, GP
通讯作者：
Siegal, GP
影响因子：
11.5
作者：
Patino-Garcia, Ana;Zalacain, Marta;Lecanda, Fernando
通讯作者：
Lecanda, Fernando