Serum microRNA expression profile: miR-1246 as a novel diagnostic and prognostic biomarker for oesophageal squamous cell carcinoma.
Serum microRNA expression profile: miR-1246 as a novel diagnostic and prognostic biomarker for oesophageal squamous cell carcinoma.
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DOI：
10.1038/bjc.2013.8
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发表时间：
2013-02-19
影响因子：
8.8
通讯作者：
Matsubara, H.
中科院分区：
文献类型：
--
作者：
Takeshita, N.;Hoshino, I.;Mori, M.;Akutsu, Y.;Hanari, N.;Yoneyama, Y.;Ikeda, N.;Isozaki, Y.;Maruyama, T.;Akanuma, N.;Komatsu, A.;Jitsukawa, M.;Matsubara, H.
最近的研究表明，在血液中可以稳定地检测到microRNAs (miRNAs)，并且可以作为有用的癌症生物标志物。我们使用从食管鳞状细胞癌（ESCC）患者或健康对照者获得的血清样本进行了miRNA阵列检测。MiR-1246在ESCC患者中升高最为显著。因此，我们选择miR-1246作为进一步分析的候选。对46例健康对照和101例ESCC患者的血清miR-1246水平进行了评估，并比较了各种临床病理特征。MiR-1246在组织、外泌体和细胞样本中的表达也被检测。血清miR-1246单独产生的接受者工作特征曲线面积为0.754，用于区分ESCC患者和健康对照的敏感性为71.3%，特异性为73.9%。血清miR-1246与TNM分期显著相关，是生存不良的最强独立危险因素（HR, 4.032； P=0.017）。与之前报道的趋势不同，miR-1246在ESCC组织样本中没有上调。此外，外泌体miR-1246不能反映起源细胞中的丰度。这些数据支持了我们的观点，即血清miR-1246具有强大的潜力作为ESCC的新型诊断和预后生物标志物，其释放机制是选择性的，与组织miRNA丰度无关。
Recent studies have demonstrated that microRNAs (miRNAs) are stably detectable in blood and can serve as useful biomarkers for cancer. We performed an miRNA array using serum samples obtained from oesophageal squamous cell carcinoma (ESCC) patients or healthy controls. MiR-1246 was the most markedly elevated in ESCC patients. Therefore, miR-1246 was selected as a candidate for further analysis. The serum miR-1246 level in 46 healthy controls and 101 ESCC patients was evaluated and compared among various clinicopathological characteristics. MiR-1246 expressions in tissue, exosomal, and cellular samples were also examined. Serum miR-1246 alone yielded an receiver-operating characteristic curve area of 0.754, with 71.3% sensitivity and 73.9% specificity for distinguishing ESCC patients from healthy controls. Serum miR-1246 was significantly correlated with the TNM stage and showed to be the strongest independent risk factor for poor survival (HR, 4.032; P=0.017). Unlike the tendency shown in previous reports, miR-1246 was not upregulated in ESCC tissue samples. Furthermore, exosomal miR-1246 did not reflect the abundance in the cell of origin. These data support our contention that serum miR-1246 has strong potential as a novel diagnostic and prognostic biomarker in ESCC, and its releasing mechanism is selective and independent of tissue miRNA abundance.
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