High-Level Acquisition of Maternal Oral Bacteria in Formula-Fed Infant Oral Microbiota.
High-Level Acquisition of Maternal Oral Bacteria in Formula-Fed Infant Oral Microbiota.
复制标题
配方奶粉喂养婴儿口腔微生物群中母体口腔细菌的高水平获取。
DOI：
10.1128/mbio.03452-21
复制
发表时间：
2022-02-22
期刊：
影响因子：
6.4
通讯作者：
Yamashita Y
中科院分区：
文献类型：
--
作者：
Kageyama S;Furuta M;Takeshita T;Ma J;Asakawa M;Yamashita Y
母亲口腔微生物的流入被认为在婴儿口腔微生物群的获得和发育中起重要作用。在这项研究中，我们检查了448对母婴在4个月检查的舌拭子样本。使用全长16S rRNA基因的PacBio单分子长读段测序和扩增子序列变体（ASV）方法测定每份样品的细菌组成。虽然婴儿口腔微生物群与母亲口腔微生物群明显不同，但与其生物学母亲共享的ASV占9.7%的中位数相对丰度（范围为0.0至99.3%），显著高于与无关母亲共享的ASV。这种共享丰度与婴儿的喂养方法密切相关，而不是与他们的分娩方式或抗生素暴露密切相关，配方奶粉喂养的婴儿比纯母乳喂养的婴儿具有更高的共享丰度。我们的研究提供了口腔细菌母婴传播的菌株水平证据，并表明配方奶粉喂养的婴儿中母体口腔细菌的定植率较高。
The influx of maternal oral microbes is considered to play an important role in the acquisition and development of infant oral microbiota. In this study, we examined tongue swab samples from 448 mother-infant pairs at 4-month checkups. The bacterial composition of each sample was determined using PacBio single-molecule long-read sequencing of the full-length 16S rRNA gene and the amplicon sequence variant (ASV) approach. Although the infant oral microbiota was distinctly different from the mother oral microbiota, ASVs shared with their biological mother accounted for a median relative abundance of 9.7% (range of 0.0 to 99.3%), which was significantly higher than that of ASVs shared with unrelated mothers. This shared abundance was strongly associated with the feeding method of infants rather than their delivery mode or antibiotic exposure, and formula-fed infants had higher shared abundance than exclusively breastfed infants. Our study presents strain-level evidence for mother-to-infant transmission of oral bacteria and suggests that colonization of maternal oral bacteria is higher in formula-fed infants.
登录
查看更多内容
影响因子：
2.9
作者：
Boustedt K;Roswall J;Dahlén G;Dahlgren J;Twetman S
通讯作者：
Twetman S
影响因子：
5.8
作者：
Gu, Zuguang;Eils, Roland;Schlesner, Matthias
通讯作者：
Schlesner, Matthias
DOI：
10.1126/science.aan4526
发表时间：
2017-10-20
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Atarashi K;Suda W;Luo C;Kawaguchi T;Motoo I;Narushima S;Kiguchi Y;Yasuma K;Watanabe E;Tanoue T;Thaiss CA;Sato M;Toyooka K;Said HS;Yamagami H;Rice SA;Gevers D;Johnson RC;Segre JA;Chen K;Kolls JK;Elinav E;Morita H;Xavier RJ;Hattori M;Honda K
通讯作者：
Honda K
影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
影响因子：
8
作者：
Huffnagle GB;Dickson RP;Lukacs NW
通讯作者：
Lukacs NW