A modified scoring system to describe gross pathology in the rabbit model of tuberculosis.
A modified scoring system to describe gross pathology in the rabbit model of tuberculosis.
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DOI：
10.1186/1471-2180-11-49
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发表时间：
2011-03-04
期刊：
影响因子：
4.2
通讯作者：
Bishai WR
中科院分区：
文献类型：
--
作者：
Jassal MS;Nedeltchev GG;Osborne J;Bishai WR
由于兔模型与人类结核病相似，因此是研究结核病发病机制的理想手段。我们以前描述的结果使用支气管镜途径感染活杆菌作为一个可靠的手段产生肺腔致敏兔。在一些动物模型中，致敏在疾病结果发展中的作用已经得到了很好的证实。我们在这里描述了不同的大体病理，由于热杀牛分枝杆菌在高剂量支气管镜下感染活杆菌之前缺乏致敏。未致敏的家兔不产生肺腔，而仅形成结核样肺炎，取代感染区正常肺实质。肺外传播在两个兔群中出现的频率和分布大致相同。值得注意的差异包括，未致敏的家兔没有肠道病变，这可能是由于没有从空洞病变中摄取痰杆菌。该实验还采用了一种改进的评分系统，该系统最初是在灵长类结核病模型中开发的，以便对尸检中观察到的结果进行量化。到目前为止，还没有这样的评分系统被用于兔模型来描述大体病理。定量方法将允许快速比较分析和标准化的胸部和肺外病理，可以评估统计意义。目的是使用这样一个评分系统作为未来所有描述结核发病各阶段大体病理的兔子研究的基础。
The rabbit model is an ideal means to study the pathogenesis of tuberculosis due to its semblance to the disease in humans. We have previously described the results using a bronchoscopic route of infection with live bacilli as a reliable means of generating lung cavities in sensitized rabbits. The role of sensitization in the development of disease outcomes has been well established in several animal models. We have described here the varying gross pathology that result from lack of sensitization with heat-killed M. bovis prior to high-dose bronchoscopic infection with live bacilli. Rabbits lacking sensitization did not generate lung cavities, but instead formed solely a tuberculoid pneumonia that replaced the normal lung parenchyma in the area of infection. Extrapulmonary dissemination was seen in approximately equal frequency and distribution in both rabbit populations. Notable differences include the lack of intestinal lesions in non-sensitized rabbits likely due to the lack of ingestion of expectorated bacilli from cavitary lesions. The experiment also employed a modified scoring system developed initially in the primate model of tuberculosis to allow for the quantification of findings observed at necropsy. To date, no such scoring system has been employed in the rabbit model to describe gross pathology. The quantitative methodology would allow for rapid comparative analyses and standardization of thoracic and extrapulmonary pathology that could be evaluated for statistical significance. The aim is to use such a scoring system as the foundation for all future rabbit studies describing gross pathology at all stages in TB pathogenesis.
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影响因子：
15.3
作者：
Lurie, MB
通讯作者：
Lurie, MB
DOI：
10.1073/pnas.150227197
发表时间：
2000-07-18
影响因子：
11.1
作者：
Kramnik, I;Dietrich, WF;Bloom, BR
通讯作者：
Bloom, BR
影响因子：
3.2
作者：
Manabe, Yukari C.;Kesavan, Anup K.;Mendez, Susana
通讯作者：
Mendez, Susana
影响因子：
15.3
作者：
RATCLIFFE, HL;WELLS, WF
通讯作者：
WELLS, WF
影响因子：
5.5
作者：
Ritz, Nicole;Connell, Tom G.;Curtis, Nigel
通讯作者：
Curtis, Nigel