Flexible and digestible wood caused by viral-induced alteration of cell wall composition.
Flexible and digestible wood caused by viral-induced alteration of cell wall composition.
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由病毒诱导的细胞壁组成改变引起的柔性和易消化的木材。
DOI：
10.1016/j.cub.2022.06.005
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发表时间：
2022-08-08
期刊：
影响因子：
9.2
通讯作者：
Turner, Simon R.
中科院分区：
文献类型：
--
作者：
Allen, Holly;Zeef, Leo;Morreel, Kris;Goeminne, Geert;Kumar, Manoj;Gomez, Leonardo D.;Dean, Andrew P.;Eckmann, Axel;Casiraghi, Cinzia;McQueen-Mason, Simon J.;Boerjan, Wout;Turner, Simon R.
木本植物材料代表了一种巨大的可再生资源，具有生产生物燃料和其他生物基产品的潜力，具有良好的二氧化碳净排放量。它的潜力已经在最近的一项研究中得到了证明，该研究从柔性可塑木材中产生了新型结构材料。苹果橡胶木（ARW）疾病是病毒感染的结果，导致木质茎表现出增加的灵活性。虽然ARW病与一种被称为苹果橡胶木病毒（ARWV）的RNA病毒的存在有关，但这种独特的症状是如何发展的还不清楚。我们证明，ARWV感染的症状出现从木质部纤维的次生细胞壁内的木质化减少，并导致木材消化率增加。相反，中层区和木质部射线细胞基本上不受感染。有症状木质部的基因表达和蛋白质组数据清楚地显示了苯丙氨酸解氨酶（PAL）的下调，该酶催化苯丙烷途径中导致木质素生物合成的第一个关键步骤。症状木质部中可溶性酚类物质的大量增加，包括木质素前体苯丙氨酸，也与PAL下调一致。ARWV感染导致许多宿主来源的病毒激活的小干扰RNA（vasiRNA）的积累。PAL衍生的vasiRNA是症状木质部中最丰富的vasiRNA之一，并且可能是PAL活性降低的原因。显然，该病毒改变木质素的机制与用于改变转基因树木中木质素的RNAi策略相似，以产生木材性质的可比改善。苹果橡胶木（ARW）症状是由木质组织中木质素减少引起的RNA序列，蛋白质组学和代谢组学表明苯丙氨酸水平降低病毒激活的小干扰RNA（vasiRNA）是响应于ARWV感染而产生的VasiRNA导致苯丙氨酸氨基于siRNA的下调苹果橡胶木（ARW）感染导致树枝变得异常灵活。艾伦等人证明症状是由木质素沉积的特定减少引起的，这是由通过苯丙氨酸解氨酶（PAL）的通量减少引起的。PAL被ARW感染后植物产生的siRNA下调。
Woody plant material represents a vast renewable resource that has the potential to produce biofuels and other bio-based products with favorable net CO2 emissions. Its potential has been demonstrated in a recent study that generated novel structural materials from flexible moldable wood. Apple rubbery wood (ARW) disease is the result of a viral infection that causes woody stems to exhibit increased flexibility. Although ARW disease is associated with the presence of an RNA virus known as apple rubbery wood virus (ARWV), how the unique symptoms develop is unknown. We demonstrate that the symptoms of ARWV infections arise from reduced lignification within the secondary cell wall of xylem fibers and result in increased wood digestibility. In contrast, the mid-lamellae region and xylem ray cells are largely unaffected by the infection. Gene expression and proteomic data from symptomatic xylem clearly show the downregulation of phenylalanine ammonia lyase (PAL), the enzyme catalyzing the first committed step in the phenylpropanoid pathway leading to lignin biosynthesis. A large increase in soluble phenolics in symptomatic xylem, including the lignin precursor phenylalanine, is also consistent with PAL downregulation. ARWV infection results in the accumulation of many host-derived virus-activated small interfering RNAs (vasiRNAs). PAL-derived vasiRNAs are among the most abundant vasiRNAs in symptomatic xylem and are likely the cause of reduced PAL activity. Apparently, the mechanism used by the virus to alter lignin exhibits similarities to the RNAi strategy used to alter lignin in genetically modified trees to generate comparable improvements in wood properties. Apple rubbery wood (ARW) symptoms are caused by decreased lignin in woody tissue RNA-seq, proteomics, and metabolomics suggest phenylalanine levels decrease Virus-activated small interfering RNAs (vasiRNAs) are generated in response to ARWV infection VasiRNAs cause siRNA-based downregulation of phenylalanine ammonia Apple rubbery wood (ARW) infections cause the branches to become abnormally flexible. Allen et al. demonstrate that the symptoms are caused by a specific decrease in lignin deposition, resulting from decreased flux through phenylalanine ammonia lyase (PAL). PAL is downregulated by siRNAs that are generated by the plant upon ARW infection.
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
14.8
作者：
Ainsworth, Elizabeth A.;Gillespie, Kelly M.
通讯作者：
Gillespie, Kelly M.
DOI：
10.1051/forest:19960604
发表时间：
1996-01-01
期刊：
ANNALES DES SCIENCES FORESTIERES
影响因子：
--
作者：
Grzeskowiak, V;Sassus, F;Fournier, M
通讯作者：
Fournier, M
影响因子：
6
作者：
Desmet S;Saeys Y;Verstaen K;Dauwe R;Kim H;Niculaes C;Fukushima A;Goeminne G;Vanholme R;Ralph J;Boerjan W;Morreel K
通讯作者：
Morreel K
影响因子：
7.4
作者：
Huang, Junli;Gu, Min;Chen, Zhixiang
通讯作者：
Chen, Zhixiang