A preclinical microbeam facility with a conventional x-ray tube.
A preclinical microbeam facility with a conventional x-ray tube.
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DOI：
10.1118/1.4966032
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发表时间：
2016-12
期刊：
影响因子：
3.8
通讯作者：
Oelfke U
中科院分区：
文献类型：
--
作者：
Bartzsch S;Cummings C;Eismann S;Oelfke U
微束放射治疗是一种创新的放射治疗方法，它使用几十微米宽的阵列和几百微米间隔的平面x射线束作为治疗场。在临床前研究中，这些电场有效地根除肿瘤，而正常组织可以有效地保留。然而，由于缺乏合适的小规模光源，阻碍了微束放射治疗的发展和临床应用。到目前为止，只有大型同步加速器才能提供适当的光束特性来生产微光束。在这项工作中，使用具有小焦点的传统x射线管和专门设计的准直器来产生用于临床前研究的微光束。在体外试验中证明了所开发的源的适用性。用放射致色膜剂量法对所产生的辐射场性质进行了表征。在20 × 20 mm2尺寸的微光束场中产生了50 μm宽、400 μm间距的微光束。峰谷剂量比从15.5到30不等，这与同步加速器获得的值相当。在微束峰值处达到了300毫戈瑞/秒的剂量率。胰腺癌细胞（Panc1）在x射线管和欧洲同步加速器的体外暴露后，DNA双链修复和细胞周期分布的分析得出了类似的结果。特别是，在微束峰区的细胞中观察到G2细胞周期阻滞的减少。在目前阶段，该来源仅限于体外应用。然而，适度修改设置可能很快允许在小鼠和大鼠体内研究。
Microbeam radiation therapy is an innovative treatment approach in radiation therapy that uses arrays of a few tens of micrometer wide and a few hundreds of micrometer spaced planar x‐ray beams as treatment fields. In preclinical studies these fields efficiently eradicated tumors while normal tissue could effectively be spared. However, development and clinical application of microbeam radiation therapy is impeded by a lack of suitable small scale sources. Until now, only large synchrotrons provide appropriate beam properties for the production of microbeams. In this work, a conventional x‐ray tube with a small focal spot and a specially designed collimator are used to produce microbeams for preclinical research. The applicability of the developed source is demonstrated in a pilot in vitro experiment. The properties of the produced radiation field are characterized by radiochromic film dosimetry. 50 μm wide and 400 μm spaced microbeams were produced in a 20 × 20 mm2 sized microbeam field. The peak to valley dose ratio ranged from 15.5 to 30, which is comparable to values obtained at synchrotrons. A dose rate of up to 300 mGy/s was achieved in the microbeam peaks. Analysis of DNA double strand repair and cell cycle distribution after in vitro exposures of pancreatic cancer cells (Panc1) at the x‐ray tube and the European Synchrotron leads to similar results. In particular, a reduced G2 cell cycle arrest is observed in cells in the microbeam peak region. At its current stage, the source is restricted to in vitro applications. However, moderate modifications of the setup may soon allow in vivo research in mice and rats.
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影响因子：
12.3
作者：
Carpenter AE;Jones TR;Lamprecht MR;Clarke C;Kang IH;Friman O;Guertin DA;Chang JH;Lindquist RA;Moffat J;Golland P;Sabatini DM
通讯作者：
Sabatini DM
DOI：
10.1016/j.ijrobp.2010.06.021
发表时间：
2010-12-01
影响因子：
7
作者：
Bouchet, Audrey;Lemasson, Benjamin;Serduc, Raphael
通讯作者：
Serduc, Raphael
DOI：
10.1073/pnas.92.19.8783
发表时间：
1995-09-12
影响因子：
11.1
作者：
SLATKIN, DN;SPANNE, P;LAISSUE, JA
通讯作者：
LAISSUE, JA
影响因子：
3.8
作者：
SLATKIN, DN;SPANNE, P;SANDBORG, M
通讯作者：
SANDBORG, M
DOI：
10.1016/0360-3016(91)90314-t
发表时间：
1991-11-01
影响因子：
7
作者：
HALL, EJ;BRENNER, DJ
通讯作者：
BRENNER, DJ