Polymorphisms influencing expression of dermonecrotic toxin in Bordetella bronchiseptica.
Polymorphisms influencing expression of dermonecrotic toxin in Bordetella bronchiseptica.
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DOI：
10.1371/journal.pone.0116604
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Horiguchi Y
中科院分区：
文献类型：
--
作者：
Okada K;Abe H;Ike F;Ogura Y;Hayashi T;Fukui-Miyazaki A;Nakamura K;Shinzawa N;Horiguchi Y
支气管败血波氏杆菌是一种致病性细菌，可引起多种哺乳动物的呼吸道感染。最近，我们测定了B的全基因组序列。支气管败血症S798菌株从感染萎缩性鼻炎的猪分离，发现4个单核苷酸多态性（SNPs）的位置-129，-72，+22，和+38的dnt编码的皮肤坏死毒素（DNT）的上游区域，当与兔分离物，RB 50相比。已知DNT参与萎缩性鼻炎中观察到的鼻甲萎缩。免疫印迹、实时荧光定量PCR和β-半乳糖苷酶报告基因检测结果表明，这些SNPs可提高dnt基因启动子活性，增强细菌产生DNT的能力。在我们实验室保存的其他猪分离株中也发现了相似或相同的SNP，所有这些猪分离株产生的DNT量都大于RB 50。我们的分析表明，在-72和+22位的四个碱基中的至少两个的取代影响dnt的启动子活性。这些结果表明，这些SNPs参与了博德特氏菌对猪疾病的致病性。
Bordetella bronchiseptica is a pathogenic bacterium causing respiratory infections in a broad range of mammals. Recently, we determined the whole genome sequence of B. bronchiseptica S798 strain isolated from a pig infected with atrophic rhinitis and found four single-nucleotide polymorphisms (SNPs) at positions -129, -72, +22, and +38 in the region upstream of dnt encoding dermonecrotic toxin (DNT), when compared with a rabbit isolate, RB50. DNT is known to be involved in turbinate atrophy observed in atrophic rhinitis. Immunoblotting, quantitative real-time PCR, and β-galactosidase reporter assay revealed that these SNPs resulted in the increased promoter activity of dnt and conferred the increased ability to produce DNT on the bacteria. Similar or identical SNPs were also found in other pig isolates kept in our laboratory, all of which produce a larger amount of DNT than RB50. Our analysis revealed that substitution of at least two of the four bases, at positions -72 and +22, influenced the promoter activity for dnt. These results imply that these SNPs are involved in the pathogenicity of bordetellae specific to pig diseases.
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DOI：
10.1128/genomea.00436-14
发表时间：
2014-05-15
期刊：
Genome announcements
影响因子：
--
作者：
Okada K;Ogura Y;Hayashi T;Abe A;Kuwae A;Horiguchi Y;Abe H
通讯作者：
Abe H
影响因子：
3.1
作者：
COWELL, JL;HEWLETT, EL;MANCLARK, CR
通讯作者：
MANCLARK, CR
影响因子：
11.4
作者：
Masuda, M;Betancourt, L;Horiguchi, Y
通讯作者：
Horiguchi, Y
影响因子：
3.1
作者：
Brockmeier, SL;Register, KB;Kunkle, RA
通讯作者：
Kunkle, RA
影响因子：
3.1
作者：
HORIGUCHI, Y;NAKAI, T;KUME, K
通讯作者：
KUME, K