Human metabolome variation along the upper intestinal tract.
Human metabolome variation along the upper intestinal tract.
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DOI：
10.1038/s42255-023-00777-z
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发表时间：
2023-05
影响因子：
20.8
通讯作者：
Fiehn, Oliver
中科院分区：
文献类型：
--
作者：
Folz, Jacob;Culver, Rebecca Neal;Morales, Juan Montes;Grembi, Jessica;Triadafilopoulos, George;Relman, David A.;Huang, Kerwyn Casey;Shalon, Dari;Fiehn, Oliver
人类饮食的大部分加工发生在小肠中。小肠中的代谢物源自宿主分泌物，加上摄入的暴露组和微生物转化。在这里，我们探讨了 15 名健康男性和女性参与者在日常消化过程中上肠腔内容物的时空变化。为此，我们使用非侵入性、可摄入的采样装置，通过结合五种质谱分析和 16S rRNA 测序来收集和分析 274 个肠道样本和 60 个相应的粪便匀浆。我们鉴定了 1,909 种代谢物，包括磺酰脂和羟基脂肪酸脂肪酸酯 (FAHFA) 脂质。我们观察到粪便和肠道代谢组存在显着差异。食物代谢物显示出膳食生物标志物的趋势、肠道中二羧酸的意外增加以及管腔酮酸与水果摄入量之间的正相关。饮食来源和微生物相关的代谢物是最大的个体差异的原因。值得注意的是，采样前 6 个月内服用过抗生素的两个人的生物活性 FAHFA 和磺脂以及其他微生物相关代谢物的水平存在很大差异。根据个体间的差异，我们将 Blautia 物种确定为参与 FAHFA 代谢的候选者。总之，在生理条件下对人体小肠和升结肠进行非侵入性体内采样揭示了饮食、宿主和微生物代谢之间的联系。作者使用非侵入性采样装置收集了 15 名健康男性和女性参与者的肠道样本，并通过质谱和 16S rRNA 测序对其进行分析。他们鉴定了整个肠道中的数千种生物分子，其中许多分子仍然未知，并突出了肠道和粪便代谢组之间的差异。
Most processing of the human diet occurs in the small intestine. Metabolites in the small intestine originate from host secretions, plus the ingested exposome and microbial transformations. Here we probe the spatiotemporal variation of upper intestinal luminal contents during routine daily digestion in 15 healthy male and female participants. For this, we use a non-invasive, ingestible sampling device to collect and analyse 274 intestinal samples and 60 corresponding stool homogenates by combining five mass spectrometry assays and 16S rRNA sequencing. We identify 1,909 metabolites, including sulfonolipids and fatty acid esters of hydroxy fatty acids (FAHFA) lipids. We observe that stool and intestinal metabolomes differ dramatically. Food metabolites display trends in dietary biomarkers, unexpected increases in dicarboxylic acids along the intestinal tract and a positive association between luminal keto acids and fruit intake. Diet-derived and microbially linked metabolites account for the largest inter-individual differences. Notably, two individuals who had taken antibiotics within 6 months before sampling show large variation in levels of bioactive FAHFAs and sulfonolipids and other microbially related metabolites. From inter-individual variation, we identify Blautia species as a candidate to be involved in FAHFA metabolism. In conclusion, non-invasive, in vivo sampling of the human small intestine and ascending colon under physiological conditions reveals links between diet, host and microbial metabolism. The authors use a non-invasive sampling device to collect intestinal samples in 15 healthy male and female participants and analyse them through mass spectrometry and 16S rRNA sequencing. They identify thousands of biomolecules throughout the intestinal tract, many of which remain unknown and highlight differences between intestinal and stool metabolomes.
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影响因子：
7.4
作者：
Bonini P;Kind T;Tsugawa H;Barupal DK;Fiehn O
通讯作者：
Fiehn O
影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
影响因子：
4.2
作者：
GIBSON, GR;CUMMINGS, JH;MACFARLANE, GT
通讯作者：
MACFARLANE, GT
影响因子：
16.6
作者：
Beaudoin GAW;Li Q;Folz J;Fiehn O;Goodsell JL;Angerhofer A;Bruner SD;Hanson AD
通讯作者：
Hanson AD
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y