Common Evolutionary Origin of Procapsid Proteases, Phage Tail Tubes, and Tubes of Bacterial Type VI Secretion Systems.
Common Evolutionary Origin of Procapsid Proteases, Phage Tail Tubes, and Tubes of Bacterial Type VI Secretion Systems.
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DOI：
10.1016/j.str.2016.08.013
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发表时间：
2016-11-01
期刊：
影响因子：
5.7
通讯作者：
Rossmann, Michael G.
中科院分区：
文献类型：
--
作者：
Fokine, Andrei;Rossmann, Michael G.
许多大型病毒，包括尾巴dsDNA噬菌体和疱疹病毒，通过形成称为前衣壳或前头的前体来组装衣壳。前头有一个由支架蛋白组成的内部核心和一个由主要衣壳蛋白形成的外壳。前头通常含有一种蛋白酶，在衣壳成熟时被激活，以破坏内核并为基因组释放空间。在这里，我们报道了噬菌体T4基因产物(GP)21的五聚体前衣壳蛋白酶的2.0分辨率结构。该结构对应于酶的预活性状态，在该状态下，其N-末端区域阻止了催化中心，表明激活机制涉及9个N-末端残基的自我切割。我们描述了T4 gp21和相关疱疹病毒蛋白水解酶的异同。我们发现gp21和疱疹病毒蛋白水解酶与形成噬菌体尾管和细菌VI型分泌系统的蛋白质具有相似性，表明它们具有共同的进化起源。Fokine和Rossmann提出了噬菌体T4衣壳组装蛋白的结构，揭示了噬菌体和疱疹病毒蛋白水解酶具有相似的折叠结构，但激活机制不同。这种结构表明，这些蛋白酶与形成噬菌体尾部管状结构和细菌VI型分泌系统的蛋白质具有共同的进化起源。
Many large viruses, including tailed dsDNA bacteriophages and herpesviruses, assemble their capsids via formation of precursors, called procapsids or proheads. The prohead has an internal core, made of scaffolding proteins, and an outer shell, formed by the major capsid protein. The prohead usually contains a protease which is activated during capsid maturation to destroy the inner core and liberate space for the genome. Here we report a 2.0 Å-resolution structure of the pentameric procapsid protease of bacteriophage T4, gene product (gp)21. The structure corresponds to the enzyme’s pre-active state in which its N-terminal region blocks the catalytic center, demonstrating that the activation mechanism involves self-cleavage of nine N-terminal residues. We describe similarities and differences between T4 gp21 and related herpesvirus proteases. We found that gp21 and the herpesvirus proteases have similarity with proteins forming the tubes of phage tails and bacterial type VI secretion systems, suggesting their common evolutionary origin. Fokine and Rossmann present the structure of the bacteriophage T4 capsid assembly protease, revealing that phage and herpesvirus proteases have similar folds but different activation mechanisms. The structure suggests that these proteases share common evolutionary origin with proteins forming the tubes of phage tails and bacterial type VI secretion systems.
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DOI：
10.1073/pnas.0400444101
发表时间：
2004-04-20
影响因子：
11.1
作者：
Fokine, A;Chipman, PR;Rossmann, MG
通讯作者：
Rossmann, MG
影响因子：
5.6
作者：
Fokine, Andrei;Zhang, Zhihong;Kanamaru, Shuji;Bowman, Valorie D.;Aksyuk, Anastasia A.;Arisaka, Fumio;Rao, Venigalla B.;Rossmann, Michael G.
通讯作者：
Rossmann, Michael G.
影响因子：
5.9
作者：
Hatfull, Graham F.;Hendrix, Roger W.
通讯作者：
Hendrix, Roger W.
DOI：
10.1002/prot.340230413
发表时间：
1995-12-01
期刊：
PROTEINS-STRUCTURE FUNCTION AND GENETICS
影响因子：
--
作者：
Janin, J;Rodier, F
通讯作者：
Rodier, F
DOI：
10.1073/pnas.1005822107
发表时间：
2010-08-10
影响因子：
11.1
作者：
Cardarelli, Lia;Pell, Lisa G.;Davidson, Alan R.
通讯作者：
Davidson, Alan R.