A Novel L-ascorbate Peroxidase 6 Gene, ScAPX6, Plays an Important Role in the Regulation of Response to Biotic and Abiotic Stresses in Sugarcane.
A Novel L-ascorbate Peroxidase 6 Gene, ScAPX6, Plays an Important Role in the Regulation of Response to Biotic and Abiotic Stresses in Sugarcane.
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一种新型的L-抗坏血酸酯过氧化物酶6基因SCAPX6在调节甘蔗中对生物和非生物胁迫的反应中起着重要作用。
DOI：
10.3389/fpls.2017.02262
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发表时间：
2017
影响因子：
5.6
通讯作者：
Su Y
中科院分区：
文献类型：
--
作者：
Liu F;Huang N;Wang L;Ling H;Sun T;Ahmad W;Muhammad K;Guo J;Xu L;Gao S;Que Y;Su Y
l -抗坏血酸过氧化物酶6基因（APX6）是清除H2O2的重要基因之一，在植物抗环境胁迫中起着至关重要的作用。在本研究中，一个新的ScAPX6基因(GenBank登录号：KT907352)是从甘蔗品种（ROC22）中获得的。生物信息学分析表明，ScAPX6全长1086 bp，编码333个氨基酸残基。亚细胞定位证实ScAPX6位于叶绿体中。表达ScAPX6的大肠杆菌BL21细胞生长增强，对铜（Cu）胁迫具有较高的耐受性。实时荧光定量PCR分析显示，ScAPX6在甘蔗的茎部和叶片中表达量最高，在根系中表达量最低。ScAPX6在水杨酸（SA）、过氧化氢（H2O2）、聚乙二醇（PEG）和氯化钠（NaCl）刺激下下调。有趣的是，在脱落酸（ABA）和茉莉酸甲酯（MeJA）胁迫下，其诱导表达量在6 h时达到最高，分别是对照的6.0倍和70.0倍。ScAPX6在烟叶中的过表达增强了烟叶对烟草病原菌茄青假单胞菌和茄蓝镰刀菌感染的抗性。这些结果表明，ScAPX6可能对ABA、MeJA和Cu有正响应，而对SA、H2O2、PEG和NaCl有负响应。从目前的研究结果来看，ScAPX6可能与甘蔗的超敏反应（hypersensitive response， HR）或免疫有关，这将为甘蔗ScAPX6的功能鉴定提供基础。
The L-ascorbate peroxidase 6 gene (APX6) is one of the most important genes for scavenging H2O2 and plays a vital role in plant resistance to environmental stresses. In this study, a novel ScAPX6 gene (GenBank Accession No. KT907352) was obtained from a sugarcane variety (ROC22). Bioinformatics analysis showed that ScAPX6 has a cDNA length of 1,086 bp and encoded 333 amino acid residues. Subcellular localization confirmed that ScAPX6 was located in the chloroplast. Enhanced growth of Escherichia coli BL21 cells that expressed ScAPX6 showed high tolerance under copper (Cu) stress. Real-time quantitative PCR analysis revealed that ScAPX6 was constitutively expressed wherein with the highest expression levels in sugarcane pith and leaf and the lowest in the root. ScAPX6 was down-regulated by salicylic acid (SA), hydrogen peroxide (H2O2), polyethylene glycol (PEG) and sodium chloride (NaCl) stimuli. Interestingly, it was significantly up-regulated under the stresses of abscisic acid (ABA) and methyl jasmonate (MeJA) wherein with the highest inducible expression levels at 6 h at 6.0- and 70.0-times higher, respectively than that of control. Overexpression of ScAPX6 in Nicotiana benthamiana leaves enhanced the resistance to the infection of tobacco pathogens Pseudomonas solanacearum and Fusarium solani var. coeruleum. These results implied that ScAPX6 might positively respond to ABA, MeJA, and Cu, but might negatively respond to the stresses of SA, H2O2, PEG, and NaCl. Keeping in view the current investigation, ScAPX6 could be associated with the hypersensitive response (HR) or immunity of sugarcane, which will provide a baseline for the function identification of sugarcane ScAPX6.
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影响因子：
4.2
作者：
D'Arcy-Lameta, A;Ferrari-Iliou, R;Zuily-Fodil, Y
通讯作者：
Zuily-Fodil, Y
影响因子：
3.5
作者：
ISHIKAWA, T;SAKAI, K;SHIGEOKA, S
通讯作者：
SHIGEOKA, S
影响因子：
4.7
作者：
Guo, Feng;Chiang, Ming-Yi;Zhang, Yu-Zhu
通讯作者：
Zhang, Yu-Zhu
影响因子：
2.1
作者：
Caverzan A;Passaia G;Rosa SB;Ribeiro CW;Lazzarotto F;Margis-Pinheiro M
通讯作者：
Margis-Pinheiro M
影响因子：
--
作者：
Guo J;Xu L;Su Y;Wang H;Gao S;Xu J;Que Y
通讯作者：
Que Y