Placental mitochondrial DNA content and particulate air pollution during in utero life.
Placental mitochondrial DNA content and particulate air pollution during in utero life.
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DOI：
10.1289/ehp.1104458
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发表时间：
2012-09
影响因子：
10.4
通讯作者：
Nawrot TS
中科院分区：
文献类型：
--
作者：
Janssen BG;Munters E;Pieters N;Smeets K;Cox B;Cuypers A;Fierens F;Penders J;Vangronsveld J;Gyselaers W;Nawrot TS
背景：研究强调颗粒物(PM)在活性氧形成和炎症中的重要性。我们假设这些过程可以影响胎盘和胎儿的线粒体功能。目的：探讨孕期PM10暴露对胎盘和脐血线粒体DNA含量的影响。方法：从174例胎盘组织和176例脐血白细胞中提取DNA。用实时荧光定量聚合酶链式反应测定线粒体DNA相对拷贝数(即线粒体DNA含量)。多元回归模型被用来联系线粒体DNA含量和孕期不同时间窗内PM10的宫内暴露。结果：在多变量调整分析中，孕期最后一个月PM10值每增加10微克/立方米，胎盘线粒体DNA含量下降16.1%[95%可信区间：-25.2，-6.0%，P=0.003]。孕晚期平均PM10暴露的相应效应大小为17.4%(95%可信区间：-31.8，-0.1%，p=0.05)。此外，我们发现，居民到主要道路的距离每增加一倍，胎盘mtDNA含量就会增加4.0%(95%可信区间：0.4，7.8%，p=0.03)。未发现脐带血mtDNA含量与PM10暴露之间的关联。结论：孕期PM10暴露与胎盘线粒体改变有关，这可能反映和加强氧化应激的产生。早期胎盘线粒体DNA含量降低的潜在健康后果必须进一步阐明。
Background: Studies emphasize the importance of particulate matter (PM) in the formation of reactive oxygen species and inflammation. We hypothesized that these processes can influence mitochondrial function of the placenta and fetus. Objective: We investigated the influence of PM10 exposure during pregnancy on the mitochondrial DNA content (mtDNA content) of the placenta and umbilical cord blood. Methods: DNA was extracted from placental tissue (n = 174) and umbilical cord leukocytes (n = 176). Relative mtDNA copy numbers (i.e., mtDNA content) were determined by real-time polymerase chain reaction. Multiple regression models were used to link mtDNA content and in utero exposure to PM10 over various time windows during pregnancy. Results: In multivariate-adjusted analysis, a 10-µg/m³ increase in PM10 exposure during the last month of pregnancy was associated with a 16.1% decrease [95% confidence interval (CI): –25.2, –6.0%, p = 0.003] in placental mtDNA content. The corresponding effect size for average PM10 exposure during the third trimester was 17.4% (95% CI: –31.8, –0.1%, p = 0.05). Furthermore, we found that each doubling in residential distance to major roads was associated with an increase in placental mtDNA content of 4.0% (95% CI: 0.4, 7.8%, p = 0.03). No association was found between cord blood mtDNA content and PM10 exposure. Conclusions: Prenatal PM10 exposure was associated with placental mitochondrial alterations, which may both reflect and intensify oxidative stress production. The potential health consequences of decreased placental mtDNA content in early life must be further elucidated.
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影响因子：
10.4
作者：
Kannan S;Misra DP;Dvonch JT;Krishnakumar A
通讯作者：
Krishnakumar A
影响因子：
5
作者：
Janssen, Stijn;Dumont, Gerwin;Mensink, Clemens
通讯作者：
Mensink, Clemens
影响因子：
4.4
作者：
Andreu, Antonio L.;Martinez, Ramiro;Garcia-Arumi, Elena
通讯作者：
Garcia-Arumi, Elena
影响因子：
168.9
作者：
Hoek, G;Brunekreef, B;van den Brandt, PA
通讯作者：
van den Brandt, PA
影响因子：
10.4
作者：
Carugno M;Pesatori AC;Dioni L;Hoxha M;Bollati V;Albetti B;Byun HM;Bonzini M;Fustinoni S;Cocco P;Satta G;Zucca M;Merlo DF;Cipolla M;Bertazzi PA;Baccarelli A
通讯作者：
Baccarelli A