Novel dual-emissive fluorescent silicon nanoparticles for detection of enzyme activity in supplements associated with lactose intolerance
Novel dual-emissive fluorescent silicon nanoparticles for detection of enzyme activity in supplements associated with lactose intolerance
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新型双发射荧光硅纳米粒子，用于检测与乳糖不耐受相关的补充剂中的酶活性
DOI：
10.1016/j.snb.2020.129164
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发表时间：
2020-11
期刊：
影响因子：
--
通讯作者：
Cen Yao
中科院分区：
文献类型：
--
作者：
Mei Jie;Bao Jian;Cheng Xia;Ren D;an;Xu Guanhong;Wei Fangdi;Sun Yong;Hu Qin;Cen Yao
采用面相一锅水热法制备了一种新型的双发射罗丹明6G修饰的纳米硅（SiNPs-Rho6G）。在320 nm激发下，发射波长为415/550 nm的双发射SiNPs-Rho6G具有强荧光和光稳定性的优点。开发了SiNPs-Rho6G，建立了一种涉及对硝基酚（PNP）的β-半乳糖苷酶（β-Gal）活性的比例荧光传感器。PNP是对硝基苯-β-d-半乳糖吡喃苷的水解产物。在β-Gal的陪同下，由于内部滤光效应，SiNPs-Rho6G在415 nm处的荧光猝灭，发生了红移，在最大激发波长320 nm下，550 nm处的荧光略有变化。β-Gal活性测定范围为1 ~ 25 U·L−1，检出限为0.72 U·L−1。该方法被进一步用于测定乳糖不耐症缓解酶制剂中β-Gal活性，具有高灵敏度和选择性。此外，该方法被证明具有成为医学、食品和药物领域各种靶分子分析平台的潜力。
A novel kind of dual-emissive Rhodamine 6G modified silicon nanoparticles (SiNPs-Rho6G) was synthesized through a facial one-pot hydrothermal method. This dual-emissive SiNPs-Rho6G with emission wavelength at 415/550 nm under 320 nm excitation possessed the advantages of strong fluorescence and photostability. SiNPs-Rho6G was developed to set up a ratiometric fluorescence sensor toward β-Galactosidase (β-Gal) activity withp-Nitrophenol (PNP) involved. PNP was the hydrolysis product ofp-Nitrophenyl-β-d-Galactopyranoside. It quenched the fluorescence of SiNPs-Rho6G at 415 nm with a red shift in the company of β-Gal due to inner filter effect, and the fluorescence at 550 nm changed slightly under the maximum excitation wavelength of 320 nm. The activity of β-Gal was determined in a range of 1–25 U·L−1with a limit of detection of 0.72 U·L−1. The proposed method was further utilized to determine the β-Gal activity in enzyme formulations for alleviating lactose intolerance with high sensitivity and selectivity. Moreover, this method was proved to have the potential of being an analysis platform for various target molecules in the field of medicine, food and drug.
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影响因子：
7.4
作者：
Zhanghua Zeng;S. Mizukami;K. Kikuchi
通讯作者：
Zhanghua Zeng;S. Mizukami;K. Kikuchi
影响因子：
4
作者：
S. Amil;E. Jiménez-Ortega;M. Ramírez-Escudero;David Talens-Perales;J. Marín-Navarro;J. Polaina;J. Sanz-Aparicio;R. Fernández-Leiro
通讯作者：
S. Amil;E. Jiménez-Ortega;M. Ramírez-Escudero;David Talens-Perales;J. Marín-Navarro;J. Polaina;J. Sanz-Aparicio;R. Fernández-Leiro
影响因子：
17.1
作者：
Dasog, Mita;Yang, Zhenyu;Regli, Sarah;Atkins, Tonya M.;Faramus, Angelique;Singh, Mani P.;Muthuswamy, Elayaraja;Kauzlarich, Susan M.;Tilley, Richard D.;Veinot, Jonathan G. C.
通讯作者：
Veinot, Jonathan G. C.
DOI：
10.1016/j.snb.2018.07.171
发表时间：
2018-11
期刊：
Sensors and Actuators B: Chemical
影响因子：
--
作者：
Eun-Joong Kim;Arup Podder;Mrinmoy Maiti;Jong min Lee;B. Chung;S. Bhuniya
通讯作者：
Eun-Joong Kim;Arup Podder;Mrinmoy Maiti;Jong min Lee;B. Chung;S. Bhuniya
影响因子：
9.5
作者：
Ahire, Jayshree H.;Chambrier, Isabelle;Chao, Yimin
通讯作者：
Chao, Yimin