Dynamic modulation of enhancer responsiveness by core promoter elements in living Drosophila embryos.
Dynamic modulation of enhancer responsiveness by core promoter elements in living Drosophila embryos.
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DOI：
10.1093/nar/gkab1177
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发表时间：
2022-01-11
影响因子：
14.9
通讯作者：
Fukaya T
中科院分区：
文献类型：
--
作者：
Yokoshi M;Kawasaki K;Cambón M;Fukaya T
增强子和核心启动子之间的调控相互作用对于发育中基因表达的时间和空间特异性至关重要。核心启动子的核心作用是响应增强子的激活线索而启动高效转录。然而，还没有系统地评估单个核心启动子元件如何影响增强子对转录爆发的诱导。在这里，我们提供的证据表明，每个核心启动子元件差异调节功能参数的转录爆发在发展中的果蝇胚胎。定量实时成像分析显示，爆发诱导的时间和连续性是核心启动子元件影响的共同调控步骤。我们进一步表明，上游TATA也影响突发幅度。另一方面，Inr、MTE和DPE主要贡献于猝发频率的调节。对配对规则基因fushi tarazu的基因组编辑分析表明，内源性TATA和DPE对于其在早期胚胎体段建立过程中的正确表达和功能都至关重要。我们认为，核心启动子元件作为一个关键的调节模块，在动物发育过程中转换增强子活性的转录动力学。
Regulatory interactions between enhancers and core promoters are fundamental for the temporal and spatial specificity of gene expression in development. The central role of core promoters is to initiate productive transcription in response to enhancer's activation cues. However, it has not been systematically assessed how individual core promoter elements affect the induction of transcriptional bursting by enhancers. Here, we provide evidence that each core promoter element differentially modulates functional parameters of transcriptional bursting in developing Drosophila embryos. Quantitative live imaging analysis revealed that the timing and the continuity of burst induction are common regulatory steps on which core promoter elements impact. We further show that the upstream TATA also affects the burst amplitude. On the other hand, Inr, MTE and DPE mainly contribute to the regulation of the burst frequency. Genome editing analysis of the pair-rule gene fushi tarazu revealed that the endogenous TATA and DPE are both essential for its correct expression and function during the establishment of body segments in early embryos. We suggest that core promoter elements serve as a key regulatory module in converting enhancer activity into transcription dynamics during animal development.
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影响因子：
4.5
作者：
Fuda NJ;Guertin MJ;Sharma S;Danko CG;Martins AL;Siepel A;Lis JT
通讯作者：
Lis JT
影响因子：
10.5
作者：
Butler, JEF;Kadonaga, JT
通讯作者：
Kadonaga, JT
影响因子：
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作者：
EDGAR, BA;WEIR, MP;KORNBERG, T
通讯作者：
KORNBERG, T
影响因子：
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作者：
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通讯作者：
Xu, Yanhui
影响因子：
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作者：
Blake, William J.;Balazsi, Gbor;Collins, James J.
通讯作者：
Collins, James J.