Rapid and simultaneous quantitation of prostanoids by UPLC-MS/MS in rat brain.
Rapid and simultaneous quantitation of prostanoids by UPLC-MS/MS in rat brain.
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DOI：
10.1016/j.jchromb.2013.11.041
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发表时间：
2014-01-15
影响因子：
3
通讯作者：
Poloyac, Samuel M.
中科院分区：
文献类型：
--
作者：
Shaik, Jafar Sadik B.;Miller, Tricia M.;Graham, Steven H.;Manole, Mioara D.;Poloyac, Samuel M.
AA由环氧合酶(COX)途径产生的代谢物，统称为前列腺素类化合物，以及由CyP450途径产生的代谢物，二十烷基类化合物，与多种神经退行性疾病和神经炎症性疾病有关。本研究建立了一种UPLC-MS/MS定量方法，同时测定大鼠脑皮质组织中前列腺素和环戊酮代谢产物等11种前列腺素类物质。线性范围为0.104~33.3ngml。所有代谢物的日间和日间变异均小于15%。12.5 ng/ml(柱上750pg)的萃取回收率和基质(去离子水)效应分别为88%~100%和3%~14%，%CV值小于20%。此外，将该方法的工艺和提取条件应用于我们以前的CYP450型二十烷基硫酸酯方法，在低浓度(0.417 ng/ml)和高浓度(8.33 ng/ml)下，在提取回收率和基质效应方面都得到了全面的提高。在大鼠脑皮质组织中，前列腺素的浓度为10.2~937pmol/g湿组织，二十烷类化合物的浓度为2.23~793pmol/g湿组织。这些数据表明，利用UPLC-MS/MS系统可以连续测定大鼠脑组织中提取的单个样本中的前列腺素和二十烷基类化合物，并且这种方法对于评估这些代谢物作为潜在的生物标志物在各种神经炎性和脑血管疾病中的作用是必要的。
The metabolites of AA produced from the cyclooxygenase (COX) pathway, collectively termed as prostanoids, and from the CYP 450 pathway, eicosanoids, have been implicated in various neurodegenerative and neuroinflammatory diseases. This study developed a quantitative UPLC-MS/MS method to simultaneously measure 11 prostanoids including prostaglandins and cyclopentenone metabolites in the rat brain cortical tissue. Linear calibration curves ranging from 0.104 to 33.3 ng/ml were validated. The inter-day and intra-day variance for all metabolites was less than 15%. The extraction recovery efficiency and matrix (deionized water) effects measured at 12.5 ng/ml (750 pg on column) ranged from 88 to 100% and 3 to 14%, respectively, with % CV values below 20%. Additionally, applying the processing and extraction conditions of this method to our previous CYP450 eicosanoids method resulted in overall improvement in extraction recovery and reduction in matrix effects at low (0.417 ng/ml) and high (8.33 ng/ml) concentrations. In rat brain cortical tissue samples, concentrations of prostanoids ranged from 10.2 to 937 pmol/g wet tissue and concentration of eicosanoids ranged from 2.23 to 793 pmol/g wet tissue. These data demonstrate that the successive measurement of prostanoids and eicosanoids from a single extracted sample of rat brain tissue can be achieved with a UPLC-MS/MS system and that this method is necessary for evaluation of these metabolites as potential biomarkers in delineating their role in various neuroinflammatory and cerebrovascular disorders.
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影响因子：
2
作者：
Masoodi, Mojgan;Nicolaou, Anna
通讯作者：
Nicolaou, Anna
影响因子：
6.1
作者：
Liu, Hao;Li, Wenjin;Ahmad, Muzamil;Miller, Tricia M.;Rose, Marie E.;Poloyac, Samuel M.;Uechi, Guy;Balasubramani, Manimalha;Hickey, Robert W.;Graham, Steven H.
通讯作者：
Graham, Steven H.
DOI：
10.1016/0090-6980(93)90118-q
发表时间：
1993-05-01
期刊：
PROSTAGLANDINS
影响因子：
--
作者：
HOFER, G;BIEGLMAYER, C;JANISCH, H
通讯作者：
JANISCH, H
影响因子：
1.8
作者：
Komaba, Junji;Matsuda, Dai;Ogawa, Mikio
通讯作者：
Ogawa, Mikio
影响因子：
7.3
作者：
CHAPPLE, DJ;DUSTING, GJ;VANE, JR
通讯作者：
VANE, JR