Discovery of J chain in African lungfish (Protopterus dolloi, Sarcopterygii) using high throughput transcriptome sequencing: implications in mucosal immunity.
Discovery of J chain in African lungfish (Protopterus dolloi, Sarcopterygii) using high throughput transcriptome sequencing: implications in mucosal immunity.
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DOI：
10.1371/journal.pone.0070650
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Salinas I
中科院分区：
文献类型：
--
作者：
Tacchi L;Larragoite E;Salinas I
J链是高等脊椎动物中负责免疫球蛋白（IG）聚合和免疫球蛋白跨粘膜表面转运的小多肽。我们确定了一个J链dipnoid鱼，非洲肺鱼（Protopterus dolloi）的转录组的高通量测序。杜氏毕赤酵母J链长161个氨基酸，含有哺乳动物J链中存在的8个Cys残基中的6个。系统发育研究将肺鱼的J链更接近于四足动物的J链，而不是腔棘鱼或护士鲨的序列。J链表达发生在所有检查的杜氏毕赤酵母免疫组织中，并且其在肠道和肾脏中响应于实验性细菌感染而增加。双荧光原位杂交显示，肠道中88.5%的IgM+细胞共表达J链，显著高于幽门前脾中的百分比。重要的是，J链表达不限于B细胞区室，因为肠上皮细胞也表达J链。这些结果改善了我们目前的J链从系统发育的角度来看。
J chain is a small polypeptide responsible for immunoglobulin (Ig) polymerization and transport of Igs across mucosal surfaces in higher vertebrates. We identified a J chain in dipnoid fish, the African lungfish (Protopterus dolloi) by high throughput sequencing of the transcriptome. P. dolloi J chain is 161 aa long and contains six of the eight Cys residues present in mammalian J chain. Phylogenetic studies place the lungfish J chain closer to tetrapod J chain than to the coelacanth or nurse shark sequences. J chain expression occurs in all P. dolloi immune tissues examined and it increases in the gut and kidney in response to an experimental bacterial infection. Double fluorescent in-situ hybridization shows that 88.5% of IgM+ cells in the gut co-express J chain, a significantly higher percentage than in the pre-pyloric spleen. Importantly, J chain expression is not restricted to the B-cell compartment since gut epithelial cells also express J chain. These results improve our current view of J chain from a phylogenetic perspective.
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DOI：
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