High-throughput sequencing of hair follicle development-related micrornas in cashmere goat at various fetal periods.
High-throughput sequencing of hair follicle development-related micrornas in cashmere goat at various fetal periods.
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绒山羊不同胎儿时期毛囊发育相关微RNA的高通量测序
DOI：
10.1016/j.sjbs.2017.12.009
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发表时间：
2018-11
影响因子：
4.4
通讯作者：
Li J
中科院分区：
文献类型：
--
作者：
Liu Y;Wang L;Li X;Han W;Yang K;Wang H;Zhang Y;Su R;Liu Z;Wang R;Wang Z;Zhao Y;Wang Z;Li J
内蒙古绒山羊以其优良的产绒性状而成为珍贵的种质遗传资源。因此，研究绒山羊绒发育机理，寻找与绒发育相关的重要候选基因具有重要意义。本研究采集了10个不同时期绒山羊胎儿皮肤样品。同时，根据前期研究结果，对平衡混合后的绒山羊胎儿皮肤毛囊起始、生长和发育3个关键时期（45、55和65 d）的侧皮肤RNA进行高通量测序。同时，将相应时期的3个样品用作生物学重复。采用高通量测序方法，获得了绒山羊不同胎龄皮肤和毛囊中microRNA和mRNA的表达数据。结果表明，相对于妊娠45天的样本，妊娠55天和66天的样本中oar-let-7和oar-miR-200家族中的microRNA显著上调。这表明它们可能是毛囊发育中的关键microRNA。
Inner Mongolia cashmere goat marks a precious gerplasm genetic resource due to its excellent cashmere traits. Therefore, it is of crucial importance to investigate the cashmere development mechanism of cashmere goat and to search for the important cashmere growth-related candidate genes. Fetal skin samples at 10 different periods of cashmere goat were collected in this research. Moreover, high-throughput sequencing was conducted on RNA samples from side skin of cashmere goat fetuses collected at three critical periods of skin hair follicle initiation, growth and development (namely, 45, 55 and 65 days) after balanced mix in line with the previous research results. Meanwhile, 3 samples at corresponding periods were used as the biological duplications. Data regarding microRNA and mRNA expression in skin and hair follicles of cashmere goats at various fetal periods were obtained using the high-throughput sequencing method. The results indicated that microRNAs in the oar-let-7 and oar-miR-200 families in 55 days and 66 days of pregnancy samples had been notably up-regulated relative to those in 45 days of pregnancy samples. This revealed that they might be the critical microRNAs in hair follicle development.
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