Environmental determinants of pyoverdine production, exploitation and competition in natural Pseudomonas communities.
Environmental determinants of pyoverdine production, exploitation and competition in natural Pseudomonas communities.
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DOI：
10.1111/1462-2920.14355
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发表时间：
2018-10
影响因子：
5.1
通讯作者：
Kümmerli R
中科院分区：
文献类型：
--
作者：
Butaitė E;Kramer J;Wyder S;Kümmerli R
许多细菌依靠铁载体的分泌来从环境中清除铁。实验室研究表明，非生物和生物因素共同决定了细菌产生多少铁载体，以及铁载体是否可以被不生产的作弊者利用，或者被生产者利用来抑制竞争对手。在这里，我们通过比较来自48个土壤和池塘群落的930株假单胞菌的铁载体焦油的产量，来探索这些见解是否适用于自然群落。我们发现，pH、铁含量、碳浓度和群落多样性决定了吡哆醇的产生水平，以及异源(非自身)吡喃类化合物对菌株的刺激或抑制程度。虽然两种生境都存在非生产型吡喃丹，但其在土壤中的流行率更高。环境和遗传分析表明，非生产者可以进化为作弊者，利用异源吡喃，但也是由于在铁可利用性增加的环境中不再使用吡喃。总体而言，我们发现环境因素比池塘中更好地解释了土壤中吡哆醇生产的菌株间差异，推测是因为池塘中的高菌株混合阻止了局部适应。我们的研究揭示了自然细菌群落的复杂性，并首次深入了解了基于铁载体的铁获取的多变量性质和环境假单胞菌之间的竞争。
Many bacteria rely on the secretion of siderophores to scavenge iron from the environment. Laboratory studies revealed that abiotic and biotic factors together determine how much siderophores bacteria make, and whether siderophores can be exploited by non-producing cheaters or be deployed by producers to inhibit competitors. Here, we explore whether these insights apply to natural communities, by comparing the production of the siderophore pyoverdine among 930 Pseudomonas strains from 48 soil and pond communities. We found that pH, iron content, carbon concentration, and community diversity determine pyoverdine production levels, and the extent to which strains are either stimulated or inhibited by heterologous (non-self) pyoverdines. While pyoverdine non-producers occurred in both habitats, their prevalence was higher in soils. Environmental and genetic analysis suggest that non-producers can evolve as cheaters, exploiting heterologous pyoverdine, but also due to pyoverdine disuse in environments with increased iron availability. Overall, we found that environmental factors explained between-strain variation in pyoverdine production much better in soils than in ponds, presumably because high strain mixing in ponds prevents local adaption. Our study sheds light on the complexity of natural bacterial communities, and provides first insights into the multivariate nature of siderophore-based iron acquisition and competition among environmental pseudomonads.
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影响因子：
5.4
作者：
Brockhurst MA;Buckling A;Racey D;Gardner A
通讯作者：
Gardner A
影响因子：
--
作者：
D'Onofrio A;Crawford JM;Stewart EJ;Witt K;Gavrish E;Epstein S;Clardy J;Lewis K
通讯作者：
Lewis K
影响因子：
14.9
作者：
Abascal F;Zardoya R;Telford MJ
通讯作者：
Telford MJ
影响因子：
8.8
作者：
Lee, William;van Baalen, Minus;Jansen, Vincent A. A.
通讯作者：
Jansen, Vincent A. A.
影响因子：
16.6
作者：
Butaitė E;Baumgartner M;Wyder S;Kümmerli R
通讯作者：
Kümmerli R