Siderophores from neighboring organisms promote the growth of uncultured bacteria.
Siderophores from neighboring organisms promote the growth of uncultured bacteria.
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DOI：
10.1016/j.chembiol.2010.02.010
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发表时间：
2010-03-26
影响因子：
--
通讯作者：
Lewis K
中科院分区：
文献类型：
--
作者：
D'Onofrio A;Crawford JM;Stewart EJ;Witt K;Gavrish E;Epstein S;Clardy J;Lewis K
大多数细菌物种不能在合成培养基上生长。许多非生长者需要来自其他细菌的生长因子，但这些化合物的性质在很大程度上是未知的。我们在这里表明，以前未培养的分离物从海洋沉积物生物膜生长在培养皿中的存在下，培养的生物体从相同的环境。一个培养的辅助菌株产生的生长因子被鉴定为新的酰基去铁胺铁载体。一组以前未培养的菌株表现出一系列的铁载体混杂生长促进。这种基于铁载体的方法使得能够培养与先前培养的微生物仅远亲的生物体。来自该栖息地的许多菌株在实验室中缺乏生长是因为无法自主产生铁载体，而由此产生的对其他微生物的化学依赖调节了环境中群落的建立。
The majority of bacterial species do not grow on synthetic media. Many non-growers require growth factors from other bacteria, but the nature of these compounds is largely unknown. We show here that previously uncultured isolates from marine sediment biofilm grow on a Petri dish in the presence of cultured organisms from the same environment. The growth factors produced by one cultured helper strain were identified as new acyl-desferrioxamine siderophores. A panel of previously uncultured isolates exhibited a range of siderophore promiscuity for growth promotion. This siderophore-based approach has enabled the culturing of organisms only distantly related to previously cultured microbes. The lack of growth in the lab for many strains from this habitat stems from an inability to autonomously produce siderophores, and the resulting chemical dependence on other microorganisms regulates community establishment in the environment.
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影响因子：
4.5
作者：
Granger, J;Price, NM
通讯作者：
Price, NM
DOI：
10.1073/pnas.1934677100
发表时间：
2003-11-25
影响因子：
11.1
作者：
Challis, GL;Hopwood, DA
通讯作者：
Hopwood, DA
影响因子：
4.4
作者：
Cifuentes, A;Antón, J;Rodríguez-Valera, F
通讯作者：
Rodríguez-Valera, F
影响因子：
3.2
作者：
LANKFORD, CE;WALKER, JR;JONES, RJ
通讯作者：
JONES, RJ
影响因子：
56.9
作者：
Kaeberlein, T;Lewis, K;Epstein, SS
通讯作者：
Epstein, SS