Effects of salicylic acid on the photosystem 2 of barley seedlings under osmotic stress

Effects of salicylic acid on the photosystem 2 of barley seedlings under osmotic stress
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渗透胁迫下水杨酸对大麦幼苗光系统2的影响

DOI:
10.1007/s10535-009-0120-1
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发表时间:
2009-11
期刊:
影响因子:
1.5
通讯作者:
Luo, M. -H.
Luo, M. -H.
中科院分区:
生物学4区
文献类型:
--
作者:
Lin, H. -H.;Du, J. -B.;Liu, W. -J.;Wang, M. -B.;Lei, T.;Yuan, S.;Chen, Y. -E.;Luo, M. -H.

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研究了外源水杨酸 (SA) 对大麦 (Hordeum vulgareL.) 幼苗光系统 2 (PS 2) 的影响。 SA 预处理提供了针对后续渗透压的保护。通过叶绿素荧光、电解质渗漏、丙二醛含量、PS 2 mRNA 和蛋白质的测定证实了 0.25 mM SA 的最高保护作用。 SA预处理增加了活性氧(ROS),立即降低了净光合速率和气孔导度,但通过激活抗氧化酶阻止了随后的渗透胁迫期间ROS的积累。 SA预处理过程中H2O2的消除抑制了几乎所有上述SA效应。因此,SA预处理主要通过ROS信号而非SA本身增强大麦幼苗的渗透胁迫耐受性。外源SA 对PS 2 的唯一SA 依赖性和ROS 独立性作用是减少非光化学猝灭。
The effects of exogenous salicylic acid (SA) on photosystem 2 (PS 2) in barley (Hordeum vulgareL.) seedlings were investigated. SA pretreatment provided protection against subsequent osmotic stress. The highest protective effect of 0.25 mM SA was confirmed by determination of chlorophyll fluorescence, electrolyte leakage, malonyldialdehyde contents, PS 2 mRNAs and proteins. SA pretreatment increased reactive oxygen species (ROS), decreased net photosynthetic rate and stomatal conductance immediately, but prevented ROS accumulation during subsequent osmotic stress by activating antioxidant enzymes. Elimination of H2O2during SA pretreatment inhibited almost all above mentioned SA effects. Therefore, SA pretreatment enhanced osmotic stress tolerance in barley seedlings mainly through ROS signals, rather than SA itself. The only SA-dependent and ROS-independent effect of exogenous SA on PS 2 was reduction of non-photochemical quenching.
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