Fine mapping and analysis of a candidate gene in tomato accession PI128216 conferring hypersensitive resistance to bacterial spot race T3
Fine mapping and analysis of a candidate gene in tomato accession PI128216 conferring hypersensitive resistance to bacterial spot race T3
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番茄登记号 PI128216 中赋予对细菌斑点小种 T3 超敏抗性的候选基因的精细定位和分析
DOI：
10.1007/s00122-011-1726-1
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发表时间：
2011-10
影响因子：
5.4
通讯作者：
Yang W.
中科院分区：
文献类型：
--
作者：
Pei C.;Wang H.;Zhang J.;Wang Y.;Francis D.M.;Yang W.
由Xanthomonas euvesicatoria、X.vesicatoria、X.perperansand X.引起的细菌斑。番茄加德纳里病(Gardneri)是番茄上最具破坏性的病害之一。生长在热带和亚热带地区。从不同种质中发掘抗性基因，并将其整合到栽培品种中，是控制该病的关键。这就是。据报道，pimpinellifoliumPI128216在11号染色体上携带Rx4基因，对T3小种具有过敏性和田间抗性。为了便于标记辅助选择在育种和基于图谱的基因克隆中的应用，建立了感病品种OH88119与抗病品种PI128216杂交的F2群体，用于Rx4基因的精细定位。利用通过不同方法开发的18个标记，我们将该基因定位在11号染色体上两个标记pcc17和pcc14之间的45.1 kb区域。根据国际番茄注释小组的注释结果，确定了一个NBS-LRR类抗性基因作为Rx4基因的候选基因。比较PI128216和OH88119的Rx4等位基因的基因组DNA序列，发现一个6个碱基的插入/缺失(Indel)和8个SNPs。利用Indel标记成功地区分了12个番茄品系的抗性和感病。这些结果将有助于Rx4基因的克隆，并为番茄育种中该基因的分子标记辅助选择提供了有用的工具。
Bacterial spot caused byXanthomonas euvesicatoria,X. vesicatoria,X. perforansandX. gardneriis one of the most destructive diseases in tomatoes (Solanum lycopersicumL.) growing in tropical and subtropical regions. Exploring resistance genes from diverse germplasm and incorporating them into cultivated varieties are critical for controlling this disease. TheS. pimpinellifoliumaccession PI128216 was reported to carry theRx4gene on chromosome 11 conferring hypersensitivity and field resistance to race T3. To facilitate the use of marker-assisted selection in breeding and map-based cloning of the gene, an F2population derived from a cross between the susceptible variety OH88119 and the resistant accession PI128216 was created for fine mapping of theRx4gene. Using 18 markers developed through various approaches, we mapped the gene to a 45.1-kb region between two markers pcc17 and pcc14 on chromosome 11. A NBS-LRR class of resistance gene was identified as the candidate for theRx4gene based on annotation results from the International Tomato Annotation Group. Comparison of the genomic DNA sequences of theRx4alleles in PI128216 and OH88119 revealed a 6-bp insertion/deletion (InDel) and eight SNPs. The InDel marker was successfully used to distinguish resistance and susceptibility in 12 tomato lines. These results will facilitate cloning theRx4gene and provide a useful tool for marker-assisted selection of this gene in tomato breeding programs.
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DOI：
10.21273/jashs.128.5.0698
发表时间：
2003-09
影响因子：
1.9
作者：
J. Scott;D. Francis;S. Miller;G. Somodi;Jeffrey B. Jones
通讯作者：
J. Scott;D. Francis;S. Miller;G. Somodi;Jeffrey B. Jones
DOI：
10.1007/978-81-322-2316-0_9
发表时间：
2015
期刊：
--
影响因子：
--
作者：
B. Singh;Ashutosh Kumar Singh
通讯作者：
B. Singh;Ashutosh Kumar Singh
DOI：
--
发表时间：
1987
期刊：
--
影响因子：
--
作者：
R. Lelliott;D. Stead
通讯作者：
R. Lelliott;D. Stead
影响因子：
1.4
作者：
Kavitha, R.;Umesha, S.
通讯作者：
Umesha, S.
DOI：
--
发表时间：
--
期刊：
--
影响因子：
--
作者：
Wei-Feng Xue;S. Homans;S. Radford
通讯作者：
Wei-Feng Xue;S. Homans;S. Radford