Scaffold protein enigma homolog 1 overcomes the repression of myogenesis activation by inhibitor of DNA binding 2.
Scaffold protein enigma homolog 1 overcomes the repression of myogenesis activation by inhibitor of DNA binding 2.
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支架蛋白谜同源物 1 克服了 DNA 结合抑制剂 2 对肌生成激活的抑制。
DOI：
10.1016/j.bbrc.2016.04.119
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发表时间：
2016
影响因子：
3.1
通讯作者：
A. Maturana
中科院分区：
文献类型：
--
作者：
Miyuki Nakatani;Jumpei Ito;R. Koyama;M. Iijima;Nobuo Yoshimoto;T. Niimi;S. Kuroda;A. Maturana
Enigma Homolog 1（ENH1）是信号蛋白和转录因子的支架蛋白。以前，我们报道ENH1过表达促进C2C12成肌细胞的分化。然而，ENH1在C2C12细胞分化中的作用的分子机制仍不清楚。ENH1通过在细胞质中隔离DNA结合蛋白2（Id2）抑制剂来抑制神经母细胞瘤细胞的增殖。Id2是基本螺旋环抑制转录因子活性的阻遏物并阻止肌生成。在此，我们发现ENH1克服了Id2对C2C12细胞肌源性分化的抑制，并且ENH1过表达促进小鼠卫星细胞活化，这是向肌源性分化的第一步。此外，我们发现ENH1与Id2在C2C12细胞和小鼠卫星细胞中相互作用。总的来说，我们的研究结果表明，ENH1通过抑制Id2活性在激活肌生成中起着重要作用。
Enigma Homolog 1 (ENH1) is a scaffold protein for signaling proteins and transcription factors. Previously, we reported that ENH1 overexpression promotes the differentiation of C2C12 myoblasts. However, the molecular mechanism underlying the role of ENH1 in the C2C12 cells differentiation remains elusive. ENH1 was shown to inhibit the proliferation of neuroblastoma cells by sequestering Inhibitor of DNA binding protein 2 (Id2) in the cytosol. Id2 is a repressor of basic Helix-Loop-Helix transcription factors activity and prevents myogenesis. Here, we found that ENH1 overcome the Id2 repression of C2C12 cells myogenic differentiation and that ENH1 overexpression promotes mice satellite cells activation, the first step toward myogenic differentiation. In addition, we show that ENH1 interacted with Id2 in C2C12 cells and mice satellite cells. Collectively, our results suggest that ENH1 plays an important role in the activation of myogenesis through the repression of Id2 activity.
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影响因子：
10.8
作者：
Maturana, Andres D.;Waelchli, Sebastien;Kuroda, Shun'ichi
通讯作者：
Kuroda, Shun'ichi
DOI：
10.1073/pnas.0600168103
发表时间：
2006-03-28
影响因子：
11.1
作者：
Lasorella, A;Iavarone, A
通讯作者：
Iavarone, A
影响因子：
3.3
作者：
Kumar, Deepak;Shadrach, Jennifer L.;Lassar, Andrew B.
通讯作者：
Lassar, Andrew B.
影响因子：
3.7
作者：
X. Tu;R. Baffa;S. Luke;M. Prisco;R. Baserga
通讯作者：
X. Tu;R. Baffa;S. Luke;M. Prisco;R. Baserga
影响因子：
2.7
作者：
YABLONKAREUVENI, Z;RIVERA, AJ
通讯作者：
RIVERA, AJ