Neomycin: An Effective Inhibitor of Jasmonate-Induced Reactions in Plants

Neomycin: An Effective Inhibitor of Jasmonate-Induced Reactions in Plants
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新霉素:茉莉酸诱导的植物反应的有效抑制剂

DOI:
10.1007/s00344-018-9884-x
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发表时间:
2018
影响因子:
4.8
通讯作者:
Mithöfer, A.
Mithöfer, A.
中科院分区:
生物学3区
文献类型:
--
作者:
Vadassery, J.;Ballhorn, D.J.;Fleming, S.R.;Mazars, C.;Pandey, S.P.;Schmidt, A.;Schuman, M.C.;Yeh, K.W.;Yilamujiang, A.;Mithöfer, A.

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茉莉酸是一种重要的植物激素,参与植物的发育过程和防御反应。许多茉莉酸介导的植物防御反应已经在模式植物中使用茉莉酸信号传导途径的突变体进行了研究。然而,在植物物种中,JA信号突变体是不可访问的,一个工具,针对JA信号的可用性是至关重要的,以调查茉莉酸依赖的过程。新霉素是一种聚阳离子氨基糖苷类抗生素,可阻断Ca2+从内部储存中释放。我们研究了新霉素对八种不同植物的不同茉莉酸诱导反应的抑制活性:烟草细胞培养中细胞内钙的测定,甘薯中孢子蛋白基因的诱导,小麦中PDF2.2基因的表达,猪笼草阿拉塔中猪笼草蛋白酶活性的测定,菜豆花外花蜜的产生,大叶杨中蜜腺的形成,云杉中萜烯的积累,和次生代谢产物的产生。我们能够表明,新霉素,一种易于管理和商业化的化合物,抑制JA介导的反应在整个植物王国。
Jasmonates are important phytohormones involved in both plant developmental processes as well as defense reactions. Many JA-mediated plant defense responses have been studied in model plants using mutants of the jasmonate signaling pathway. However, in plant species where JA-signaling mutants are not accessible, the availability of a tool targeting JA signaling is crucial to investigate jasmonate-dependent processes. Neomycin is a poly-cationic aminoglycoside antibiotic that blocks the release of Ca2+from internal stores. We examined the inhibitory activities of neomycin on different jasmonate-inducible responses in eight different plant species: Intracellular calcium measurements inNicotiana tabacumcell culture, Sporamin gene induction inIpomoea batatas, PDF2.2gene expression inTriticum aestivum, Nepenthesin protease activity measurement inNepenthes alata, extrafloral nectar production inPhaseolus lunatus, nectary formation inPopulus trichocarpa, terpene accumulation inPicea abies, and secondary metabolite generation inNicotiana attenuata. We are able to show that neomycin, an easily manageable and commercially available compound, inhibits JA-mediated responses across the plant kingdom.
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