The nucleus together with the cytosol generates patterns of specific cellular calcium signatures in tobacco suspension culture cells.
The nucleus together with the cytosol generates patterns of specific cellular calcium signatures in tobacco suspension culture cells.
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细胞核与细胞质一起在烟草悬浮培养细胞中产生特定细胞钙特征的模式。
DOI：
10.1054/ceca.2001.0250
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发表时间：
2001
期刊：
影响因子：
4
通讯作者：
C. Mazars
中科院分区：
文献类型：
--
作者：
N. Pauly;M. Knight;P. Thuleau;A. Graziana;S. Muto;R. Ranjeva;C. Mazars
植物细胞悬浮培养物通过改变细胞游离钙水平对渗透压变化作出反应。利用水母发光蛋白重组方法，我们已经测量了空间和时间的特点，钙信号的细胞核和细胞质的BY-2烟草悬浮细胞的挑战与低或高渗休克。我们在这里表明，核隔室有助于与细胞质一起产生钙信号模式，区分低渗和高渗治疗，即从张力膨压。我们还表明，在细胞核和细胞质中的钙反应的差异调制的强度和性质的高渗处理。我们的结论是，定性和定量的变化，如渗透压变化的外部刺激的参数转换成钙的签名，其独特的时间和亚细胞特征，涉及细胞核和胞质溶胶。我们的研究结果说明了植物细胞中钙信号的多功能性。除了细胞的生理“地址”之外，钙信号的区室化可能是编码响应特异性的重要参数。
Plant cell suspension cultures respond to osmotic changes by alterations in levels of free cellular calcium. Using the aequorin recombinant method, we have measured the spatial and temporal characteristics of calcium signatures in the nucleus and the cytosol of BY-2 tobacco suspension cells challenged with hypo- or hyper-osmotic shock. We show here that the nuclear compartment contributes together with the cytosol to produce calcium signal patterns that discriminate hypo- from hyper-osmotic treatments, i.e. turgor from tension. We also demonstrate that calcium responses in the nucleus and the cytosol are differentially modulated by the strength and the nature of hyper-osmotic treatments. We conclude that qualitative and quantitative changes in the parameters of an external stimulus such as osmotic changes are converted into calcium signatures, distinctive in their temporal and subcellular characteristics, involving both the nucleus and the cytosol. Our results illustrate the versatility of calcium signaling in plant cells. In addition to the physiological 'address' of the cell, the compartmentation of the calcium signal is probably an important parameter in encoding response specificity.
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DOI：
10.1073/pnas.94.3.849
发表时间：
1997-02-04
影响因子：
11.1
作者：
Maniotis, AJ;Chen, CS;Ingber, DE
通讯作者：
Ingber, DE
DOI：
10.1046/j.1365-313x.1993.t01-4-00999.x
发表时间：
1993
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Jiu-ping Ding;B. Pickard
通讯作者：
Jiu-ping Ding;B. Pickard
影响因子：
56.9
作者：
JOHNSON, CH;KNIGHT, MR;TREWAVAS, A
通讯作者：
TREWAVAS, A
影响因子：
56.9
作者：
Xian-Cheng Yang;Frederick Sachs
通讯作者：
Xian-Cheng Yang;Frederick Sachs
影响因子：
56.9
作者：
Allen, GJ;Chu, SP;Schroeder, JI
通讯作者：
Schroeder, JI