Mutation in BrGGL7 gene encoding a GDSL esterase / lipase causes male sterility in Chinese cabbage (Brassica rapa L. ssp. pekinensis)
Mutation in BrGGL7 gene encoding a GDSL esterase / lipase causes male sterility in Chinese cabbage (Brassica rapa L. ssp. pekinensis)
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编码 GDSL 酯酶/脂肪酶的 BrGGL7 基因突变导致大白菜（Brassica rapa L. ssp. pekinensis）雄性不育
DOI：
10.1007/s00122-022-04165-1
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发表时间：
2022-07
影响因子：
5.4
通讯作者：
Hui Feng
中科院分区：
文献类型：
--
作者：
Ying Zhao;Shengnan Huang;Jiaqi Zou;Shiyao Dong;Nan Wang;Hui Feng
MutMap and KASP analyses revealed that the BrGGL7 gene is responsible for the male-sterile trait of ftms1 in Chinese cabbage, with functional verification in Arabidopsis. The application of a male-sterile line is an ideal approach of hybrid seed production in Chinese cabbage. In this study, we obtained a male-sterile mutant (ftms1) from the double haploid line 'FT' using ethyl methane sulfonate (EMS) mutagenesis. The mutant was completely sterile due to abnormal enlargement and vacuolization of the tapetum cells. A single recessive nuclear gene was found to control male sterility in the mutant, while MutMap and KASP analyses identified BraA05g022470.3C (BrGGL7), which encodes a GDSL esterase / lipase, as the candidate mutant gene. A single nucleotide substitution from C to T occurred within the domain of BrGGL7 in ftms1, resulting in premature translation termination in the fourth exon. Meanwhile, qRT-PCR analysis indicated that BrGGL7 was prominently expressed in the anothers, and expression was greater in the wild-type 'FT' than ftms1. Genetic complementation of the orthologous Arabidopsis ggl7 mutant further confirmed the role of BrGGL7 in pollen development. These findings suggest that BrGGL7 plays a fundamental role in pollen formation, providing important insight into the molecular mechanisms underlying male sterility in Chinese cabbage.
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影响因子：
5.1
作者：
Ma Y;Kang J;Wu J;Zhu Y;Wang X
通讯作者：
Wang X
甘蓝(Brassica rapa L. ssp)雄性不育突变体基因ftms的定位。
影响因子：
5.4
作者：
Chong Tan;Zhiyong Liu;Shengnan Huang;Hui Feng
通讯作者：
Hui Feng
影响因子：
7.2
作者：
Cecchetti, Valentina;Altamura, Maria Maddalena;Cardarelli, Maura
通讯作者：
Cardarelli, Maura
DOI：
10.1016/j.bbrc.2020.03.179
发表时间：
2020-04
影响因子：
3.1
作者：
D. Tsugama;K. Fujino;Shenkui Liu;T. Takano
通讯作者：
D. Tsugama;K. Fujino;Shenkui Liu;T. Takano
甘蓝型油菜花粉外壁形成和绒毡层发育需要两个重复的 CYP704B1 同源基因 BnMs1 和 BnMs2
影响因子：
7.2
作者：
Yi, Bin;Zeng, Fangqin;Fu, Tingdong
通讯作者：
Fu, Tingdong