Identification of tapetum-specific genes by comparing global gene expression of four different male sterile lines in Brassica oleracea.
Identification of tapetum-specific genes by comparing global gene expression of four different male sterile lines in Brassica oleracea.
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DOI：
10.1007/s11103-015-0287-0
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发表时间：
2015-04
影响因子：
5.1
通讯作者：
Wang X
中科院分区：
文献类型：
--
作者：
Ma Y;Kang J;Wu J;Zhu Y;Wang X
绒毡层为花粉发育提供必需的酶和营养物质，在花药发育中起着重要作用。然而，由于绒毡层细胞难以与其他花药组织分离，因此很难大规模地鉴定绒毡层特异基因。本研究通过比较4个甘蓝雄性不育系的基因表达谱，鉴定了绒毡层特异基因。透射电镜观察表明，4个不育系的败育表型表现为绒毡层和花粉发育缺陷，但花药壁发育正常。这些绒毡层在整个花药发育过程中表现出连续的缺陷特征。对花蕾的转录组进行了分析，涵盖了花药发育的所有阶段，并进行了探索绒毡层发育相关基因的生物信息学分析。我们鉴定了1,005个在至少一个MS系中差异表达的基因，104个是非花粉表达基因（NPGs）。考虑到花药壁在所有四个MS系中正常发育，大多数鉴定的NPG是绒毡层特异性基因。在这104个NPG中，有22个基因被报道与绒毡层的发育有关。我们进一步根据MS缺陷将表达的NPG分为不同的发育阶段。本研究不仅为B绒毡层发育的研究提供了重要的资料，甘蓝，但也适用于其他相关物种的遗传途径研究。本文的在线版本（doi：10.1007/s11103-015-0287-0）包含补充材料，可供授权用户使用。
The tapetum plays an important role in anther development by providing necessary enzymes and nutrients for pollen development. However, it is difficult to identify tapetum-specific genes on a large-scale because of the difficulty of separating tapetum cells from other anther tissues. Here, we reported the identification of tapetum-specific genes by comparing the gene expression patterns of four male sterile (MS) lines of Brassica oleracea. The abortive phenotypes of the four MS lines revealed different defects in tapetum and pollen development but normal anther wall development when observed by transmission electron microscopy. These tapetum displayed continuous defective characteristics throughout the anther developmental stages. The transcriptome from flower buds, covering all anther developmental stages, was analyzed and bioinformatics analyses exploring tapetum development-related genes were performed. We identified 1,005 genes differentially expressed in at least one of the MS lines and 104 were non-pollen expressed genes (NPGs). Most of the identified NPGs were tapetum-specific genes considering that anther walls were normally developed in all four MS lines. Among the 104 NPGs, 22 genes were previously reported as being involved in tapetum development. We further separated the expressed NPGs into different developmental stages based on the MS defects. The data obtained in this study are not only informative for research on tapetum development in B. oleracea, but are also useful for genetic pathway research in other related species. The online version of this article (doi:10.1007/s11103-015-0287-0) contains supplementary material, which is available to authorized users.
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影响因子：
7.4
作者：
Hooker, TS;Millar, AA;Kunst, L
通讯作者：
Kunst, L
影响因子：
7.4
作者：
Honys, D;Twell, D
通讯作者：
Twell, D
影响因子：
12.3
作者：
Honys D;Twell D
通讯作者：
Twell D
影响因子：
--
作者：
Amagai, M;Ariizumi, T;Watanabe, M
通讯作者：
Watanabe, M
影响因子：
4.9
作者：
Fujii, Sota;Yamada, Mari;Toriyama, Kinya
通讯作者：
Toriyama, Kinya