Applications of next-generation sequencing technologies to diagnostic virology.
Applications of next-generation sequencing technologies to diagnostic virology.
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DOI：
10.3390/ijms12117861
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发表时间：
2011
影响因子：
5.6
通讯作者：
Palù G
中科院分区：
文献类型：
--
作者：
Barzon L;Lavezzo E;Militello V;Toppo S;Palù G
被称为“下一代”测序（NGS）的新型DNA测序技术提供了高速度和高通量，其可以产生大量序列，在研究和诊断环境中具有许多可能的应用。在这篇文章中，我们概述了NGS在诊断病毒学中的许多应用。NGS技术已用于高通量全病毒基因组测序，如新型流感病毒的测序，用于检测宿主内病毒基因组变异性和进化，如人类免疫缺陷病毒和人类丙型肝炎病毒准种的研究，以及低丰度抗病毒药物耐药性突变的监测。NGS技术已应用于基于宏基因组学的策略，用于检测意外的疾病相关病毒和发现新的人类病毒，包括癌症相关病毒。最后，基于NGS的宏基因组学已经描述了健康和疾病条件下的人类病毒组。
Novel DNA sequencing techniques, referred to as “next-generation” sequencing (NGS), provide high speed and throughput that can produce an enormous volume of sequences with many possible applications in research and diagnostic settings. In this article, we provide an overview of the many applications of NGS in diagnostic virology. NGS techniques have been used for high-throughput whole viral genome sequencing, such as sequencing of new influenza viruses, for detection of viral genome variability and evolution within the host, such as investigation of human immunodeficiency virus and human hepatitis C virus quasispecies, and monitoring of low-abundance antiviral drug-resistance mutations. NGS techniques have been applied to metagenomics-based strategies for the detection of unexpected disease-associated viruses and for the discovery of novel human viruses, including cancer-related viruses. Finally, the human virome in healthy and disease conditions has been described by NGS-based metagenomics.
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影响因子：
3.8
作者：
Bishop-Lilly KA;Turell MJ;Willner KM;Butani A;Nolan NM;Lentz SM;Akmal A;Mateczun A;Brahmbhatt TN;Sozhamannan S;Whitehouse CA;Read TD
通讯作者：
Read TD
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作者：
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作者：
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通讯作者：
Eloit, Marc
DOI：
10.1073/pnas.0504666102
发表时间：
2005-09-06
影响因子：
11.1
作者：
Allander, T;Tammi, MT;Andersson, B
通讯作者：
Andersson, B
影响因子：
4.8
作者：
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通讯作者：
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