Specificity of Interactions between Components of Two Zinc ABC Transporters in Paracoccus denitrificans.
Specificity of Interactions between Components of Two Zinc ABC Transporters in Paracoccus denitrificans.
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DOI：
10.3390/ijms21239098
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发表时间：
2020-11-30
影响因子：
5.6
通讯作者：
Yukl ET
中科院分区：
文献类型：
--
作者：
Meléndez AB;Valencia D;Yukl ET
细菌ATP结合盒（ABC）转运体介导许多底物的内流。簇A-I ABC转运蛋白负责对必需金属锌、锰或铁的特定摄取，使它们在金属有限的环境中生存所必需，对病原体来说，这种环境包括动物宿主。在反硝化副球菌中，存在两个锌ABC转运系统：ZnuABC和AztABCD，它们在锌限制条件下具有明显的冗余功能。在同一生物体中，两个锌ABC转运体系统的不寻常存在使得研究溶质结合蛋白（SBP）与其同源渗透酶之间相互作用的特异性成为可能。我们还评估了舒张压中柔性环特征在渗透酶结合和锌运输中的作用。结果表明，SBP -通透酶相互作用具有高度特异性，不需要SBP的柔性环特征。我们还提出了特征集群a - i sbp属性的扩展表和多序列比对，突出了保守特征。通过这一分析，发现了一个与ABC转运蛋白相关的明显新的结合蛋白家族。在几种人类病原体中存在的同源物提高了将其用作开发新的抗菌疗法的目标的可能性。
Bacterial ATP binding cassette (ABC) transporters mediate the influx of numerous substrates. The cluster A-I ABC transporters are responsible for the specific uptake of the essential metals zinc, manganese or iron, making them necessary for survival in metal-limited environments, which for pathogens include the animal host. In Paracoccus denitrificans, there are two zinc ABC transporter systems: ZnuABC and AztABCD with apparently redundant functions under zinc-limited conditions. The unusual presence of two zinc ABC transporter systems in the same organism allowed for the investigation of specificity in the interaction between the solute binding protein (SBP) and its cognate permease. We also assessed the role of flexible loop features in the SBP in permease binding and zinc transport. The results indicate that the SBP–permease interaction is highly specific and does not require the flexible loop features of the SBP. We also present an expanded table of the properties of characterized cluster A-I SBPs and a multiple sequence alignment highlighting the conserved features. Through this analysis, an apparently new family of binding proteins associated with ABC transporters was identified. The presence of homologues in several human pathogens raises the possibility of using it as a target for the development of new antimicrobial therapies.
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影响因子：
7.8
作者：
Kehl-Fie, Thomas E.;Skaar, Eric P.
通讯作者：
Skaar, Eric P.
DOI：
10.1016/j.bbapap.2015.04.015
发表时间：
2015-08
影响因子：
3.2
作者：
Gerlt, John A.;Bouvier, Jason T.;Davidson, Daniel B.;Imker, Heidi J.;Sadkhin, Boris;Slater, David R.;Whalen, Katie L.
通讯作者：
Whalen, Katie L.
影响因子：
64.8
作者：
Hollenstein, Kaspar;Frei, Dominik C.;Locher, Kaspar P.
通讯作者：
Locher, Kaspar P.
影响因子：
2.1
作者：
Garrido, ME;Bosch, M;Barbé, J
通讯作者：
Barbé, J
影响因子：
48
作者：
Gibson, Daniel G.;Young, Lei;Smith, Hamilton O.
通讯作者：
Smith, Hamilton O.