Identification of the weevil immune genes and their expression in the bacteriome tissue.
Identification of the weevil immune genes and their expression in the bacteriome tissue.
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DOI：
10.1186/1741-7007-6-43
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发表时间：
2008-10-16
期刊：
影响因子：
5.4
通讯作者：
Heddi A
中科院分区：
文献类型：
--
作者：
Anselme C;Pérez-Brocal V;Vallier A;Vincent-Monegat C;Charif D;Latorre A;Moya A;Heddi A
与互利共生的细胞内细菌（内共生体）的持续感染在昆虫中有很好的代表性，并被认为是进化的驱动力。然而，尽管在过去的几十年中已经对致病关系进行了很好的研究，但对宿主免疫系统对内共生体的识别以及限制其感染携带细菌的宿主组织（细菌组）的机制知之甚少。为了研究细菌组的免疫特异性，我们首先利用抑制性消减杂交（SSH）技术鉴定了玉米象免疫相关基因，然后利用RACE-PCR实验分析了它们的全长编码序列。然后，我们测量了细菌组中的免疫基因表达，以及在S.玉米初级内共生体（SZPE）注射到血淋巴中，以考虑细菌组免疫特异性和昆虫对共生体的体液反应的问题。我们表明，幼虫的挑战与内共生体的结果在一个显着的诱导抗菌肽基因，提供证据表明，细菌组外，SZPE被认为是微生物入侵者的主机。在细菌组中，基因表达分析显示来自鞘翅素家族的一种抗菌肽的过表达，并且有趣的是，与被描述为免疫调节剂的基因（即，PGRP-LB，Tollip）的同源物也显示在细菌组中高度表达。目前的数据提供了昆虫细菌组中免疫基因表达的第一个描述。与昆虫对SZPE的体液反应相比，细菌组在这项工作中所研究的基因中表达很少。这种局部免疫基因表达可能有助于维持细菌组中的内共生体，并防止其侵入昆虫组织。对鞘翅素、PGRP和Tollip基因的进一步研究将阐明宿主免疫系统在维持和调节内共生中的作用。
Persistent infections with mutualistic intracellular bacteria (endosymbionts) are well represented in insects and are considered to be a driving force in evolution. However, while pathogenic relationships have been well studied over the last decades very little is known about the recognition of the endosymbionts by the host immune system and the mechanism that limits their infection to the bacteria-bearing host tissue (the bacteriome). To study bacteriome immune specificity, we first identified immune-relevant genes of the weevil Sitophilus zeamais by using suppressive subtractive hybridization (SSH) and then analyzed their full-length coding sequences obtained by RACE-PCR experiments. We then measured immune gene expression in the bacteriome, and in the aposymbiotic larvae following S. zeamais primary endosymbiont (SZPE) injection into the hemolymph, in order to consider the questions of bacteriome immune specificity and the insect humoral response to symbionts. We show that larval challenge with the endosymbiont results in a significant induction of antibacterial peptide genes, providing evidence that, outside the bacteriome, SZPE are recognized as microbial intruders by the host. In the bacteriome, gene expression analysis shows the overexpression of one antibacterial peptide from the coleoptericin family and, intriguingly, homologs to genes described as immune modulators (that is, PGRP-LB, Tollip) were also shown to be highly expressed in the bacteriome. The current data provide the first description of immune gene expression in the insect bacteriome. Compared with the insect humoral response to SZPE, the bacteriome expresses few genes among those investigated in this work. This local immune gene expression may help to maintain the endosymbiont in the bacteriome and prevent its invasion into insect tissues. Further investigations of the coleoptericin, the PGRP and the Tollip genes should elucidate the role of the host immune system in the maintenance and regulation of endosymbiosis.
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影响因子：
6.7
作者：
Bischoff V;Vignal C;Duvic B;Boneca IG;Hoffmann JA;Royet J
通讯作者：
Royet J
DOI：
10.1073/pnas.182213299
发表时间：
2002-09-17
影响因子：
11.1
作者：
Dale, C;Plague, GR;Moran, NA
通讯作者：
Moran, NA
影响因子：
10.7
作者：
Lazzaro, BP;Clark, AG
通讯作者：
Clark, AG
影响因子：
3.8
作者：
Charles, H;Condemine, G;Nardon, P
通讯作者：
Nardon, P
影响因子：
4.4
作者：
Kimbell, JR;McFall-Ngai, MJ
通讯作者：
McFall-Ngai, MJ