Self-assembled supramolecular immunomagnetic nanoparticles through π-π stacking strategy for the enrichment of circulating tumor cells.
Self-assembled supramolecular immunomagnetic nanoparticles through π-π stacking strategy for the enrichment of circulating tumor cells.
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DOI：
10.1093/rb/rbad016
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发表时间：
2023
影响因子：
6.7
通讯作者：
Wu Y
中科院分区：
文献类型：
--
作者：
Mao Y;Zhang Y;Yu Y;Zhu N;Zhou X;Li G;Yi Q;Wu Y
免疫磁珠(IMB)由于具有高特异性的靶向结合和快速方便的分离操作，被广泛应用于循环肿瘤细胞(CTCs)的捕获和检测。为了构建IMB，通常进行表面改性以使磁芯功能化(例如，Fe3O4纳米颗粒)，所采用的表面改性策略通常反过来影响所制备的IMB的结构和功能。与已有的工作不同，我们提出了使用超分子逐层自组装策略来构建IMB。一般来说，由于π-π的堆积作用，聚多巴胺、氧化石墨烯和分子胶γ-氧代-1-丙二烯丁酸依次自组装到Fe_3O_4纳米粒子上，从而实现不同功能组分在磁核上的整合，制备自组装的超分子免疫磁珠。ASIMBS对模型CTCs具有较高的敏感性、特异性和良好的生物相容性，对阴性细胞的非特异性吸附较低(∼分别为MCF7细胞93%和Jurkat细胞17%)。同时，ASIMBS在从大量干扰性Jurkat细胞或小鼠全血中筛选罕见的MCF-7细胞具有显著的潜力，捕获率为75-100%和20-90%。捕获的细胞可以直接再培养，不需要任何处理，这表明ASIMBS在临床应用中具有很大的体外检测能力。
Owing to their high-specific binding toward targets as well as fast and convenient separation operations, immunomagnetic beads (IMBs) are widely used in the capture and detection of circulating tumor cells (CTCs). To construct the IMBs, surface modifications are generally performed to functionalize the magnetic cores (e.g. Fe3O4 nanoparticles), and the employed surface modification strategies normally influence the structure and functions of the prepared IMBs in return. Different from the existing work, we proposed the use of supramolecular layer-by-layer (LBL) self-assembly strategy to construct the IMBs. In general, owing to the π–π stacking interactions, the polydopamine, graphene oxide and ‘molecular glue’ γ-oxo-1-pyrenebutyric acid were self-assembled on Fe3O4 nanoparticles sequentially, thereby accomplishing the integration of different functional components onto magnetic cores to prepare the self-assembled supramolecular immunomagnetic beads (ASIMBs). The ASIMBs showed high sensitivity, specificity and good biocompatibility to the model CTCs and low nonspecific adsorption to the negative cells (∼93% for MCF-7 cells and 17% for Jurkat cells). Meanwhile, ASIMBs possessed a remarkable potential to screen the rare MCF-7 cells out of large amounts of interfering Jurkat cells with the capture efficiency of 75–100% or out of mouse whole blood with the capture efficiency of 20–90%. The captured cells can be further recultured directly without any more treatment, which showed huge applicability of the ASIMBs for in vitro detection in clinical practices.
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DOI：
10.1126/science.1235226
发表时间：
2013-09-13
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Plaks V;Koopman CD;Werb Z
通讯作者：
Werb Z
影响因子：
6.1
作者：
Kang, Joo H.;Krause, Silva;Ingber, Donald E.
通讯作者：
Ingber, Donald E.
影响因子：
6.7
作者：
Lai, Chian-Hui;Tsai, Wen-Sy;Chang, Ying-Chih
通讯作者：
Chang, Ying-Chih
基于石墨碳氮化物多巴胺自聚合的荧光可调探针抗氧化能力测定
影响因子：
8.4
作者：
Liu, Jing-Jing;Chen, Zhi-Tao;Yao, Jian-Nian
通讯作者：
Yao, Jian-Nian
影响因子：
4.1
作者：
Mohamed，Hisham;Murray，Megan;Caggana，Michele
通讯作者：
Caggana，Michele