Painless Photodynamic Therapy Triggers Innate and Adaptive Immune Responses in a Murine Model of UV-induced Squamous Skin Pre-cancer.
Painless Photodynamic Therapy Triggers Innate and Adaptive Immune Responses in a Murine Model of UV-induced Squamous Skin Pre-cancer.
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DOI：
10.1111/php.13350
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发表时间：
2021-05
影响因子：
3.3
通讯作者：
Maytin, Edward V.
中科院分区：
文献类型：
--
作者：
Anand, Sanjay;Govande, Mukul;Yasinchak, Anton;Heusinkveld, Lauren;Shakya, Sajina;Fairchild, Robert L.;Maytin, Edward V.
Painless photodynamic therapy (p-PDT), which involves application of photosensitizer and immediate exposure to light to treat actinic keratosis (AK) in patients, causes negligible pain on the day of treatment but leads to delayed inflammation and effective lesion clearance [Kaw et al. (2020) J Am Acad Dermatol, 82, 862–868]. To better understand how p-PDT works, hairless mice with UV-induced AK were treated with p-PDT and monitored for 2 weeks. Lesion clearance after p-PDT was similar to clearance after conventional PDT (c-PDT). However, lesion biopsies showed minimal cell death and less production of reactive oxygen species (ROS) in p-PDT-treated than in c-PDT-treated lesions. Interestingly, p-PDT triggered vigorous recruitment of immune cells associated with innate immunity. Neutrophils (Ly6G+) and macrophages (F4/80+) appeared at 4 h and peaked at 24 h after p-PDT. Damage Associated Molecular Patterns (DAMPs) including calreticulin, HMGB1, and HSP70, were expressed at maximum levels around 24 h post p-PDT. Total T-cells (CD3+) were increased at 24 h, whereas large increases in cytotoxic (CD8+) and regulatory (Foxp3+) T-cells were observed at 1- and 2- weeks post p-PDT. In summary, the ability of p-PDT to eliminate AK lesions, despite very little overt cellular damage, appears to involve stimulation of a local immune response.
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DOI：
10.3322/caac.20114
发表时间：
2011-07
期刊：
CA: a cancer journal for clinicians
影响因子：
--
作者：
Agostinis P;Berg K;Cengel KA;Foster TH;Girotti AW;Gollnick SO;Hahn SM;Hamblin MR;Juzeniene A;Kessel D;Korbelik M;Moan J;Mroz P;Nowis D;Piette J;Wilson BC;Golab J
通讯作者：
Golab J
影响因子：
7.2
作者：
Garg, Abhishek D.;Nowis, Dominika;Agostinis, Patrizia
通讯作者：
Agostinis, Patrizia
影响因子：
2.6
作者：
Gholam, Patrick;Bosselmann, Ina;Dick, Julika
通讯作者：
Dick, Julika
影响因子：
3.7
作者：
Anand S;Ebner J;Warren CB;Raam MS;Piliang M;Billings SD;Maytin EV
通讯作者：
Maytin EV
影响因子：
11.2
作者：
Anand S;Wilson C;Hasan T;Maytin EV
通讯作者：
Maytin EV