Role of placental inflammatory mediators and growth factors in patients with rheumatic diseases with a focus on systemic sclerosis.
Role of placental inflammatory mediators and growth factors in patients with rheumatic diseases with a focus on systemic sclerosis.
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DOI：
10.1093/rheumatology/keaa782
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发表时间：
2021-07-01
期刊：
影响因子：
--
通讯作者：
Graham GJ
中科院分区：
文献类型：
--
作者：
Motta F;Codullo V;Ramoni V;Cesari S;Ferrario G;Fiandrino G;Beneventi F;Rampello S;Johnsson H;Montecucco C;Graham GJ
SSc妊娠的产科并发症风险增加。对潜在的胎盘变化知之甚少。本研究旨在更好地了解SSc胎盘的病理变化和炎症的作用。将SSc胎盘中的白细胞浸润、炎症介质和非典型趋化因子受体2（ACKR 2）表达与其他风湿性疾病（ORD）和健康对照（HC）进行比较。对8例妊娠SSc患者与16例与胎龄匹配的ORD和16例HC患者进行了病例对照研究。收集临床数据。获得胎盘用于组织病理学分析和免疫组织化学（CD 3、CD 20、CD 11 c、CD 68、ACKR 2）。通过qPCR分析来自4个SSc、8个ORD和8个HC的样品的ACKR 2表达，并通过多重测定分析参与血管生成和炎症的细胞因子、趋化因子和生长因子。胎盘CD 3、CD 68和CD 11细胞数量在风湿性疾病患者（SSc+ORD）中显著高于HC。风湿性疾病患者组（SSc+ORD）中肝细胞生长因子（HGF）显著高于HC组，而趋化因子（C-C基序）配体5（CCL 5）在SSc组中显著高于ORD和HC组。CCL 5水平与所有局部炎性细胞的数量直接相关，并且更高的水平与组织学绒毛炎相关。炎症性改变是风湿性疾病患者胎盘的特征，并可能使这些患者易于发生产科并发症。
Pregnancy in SSc is burdened with an increased risk of obstetric complications. Little is known about the underlying placental alterations. This study aimed to better understand pathological changes and the role of inflammation in SSc placentas. Leucocyte infiltration, inflammatory mediators and atypical chemokine receptor 2 (ACKR2) expression in SSc placentas were compared with those in other rheumatic diseases (ORD) and healthy controls (HC). A case–control study was conducted on eight pregnant SSc patients compared with 16 patients with ORD and 16 HC matched for gestational age. Clinical data were collected. Placentas were obtained for histopathological analysis and immunohistochemistry (CD3, CD20, CD11c, CD68, ACKR2). Samples from four SSc, eight ORD and eight HC were analysed by qPCR for ACKR2 expression and by multiplex assay for cytokines, chemokines and growth factors involved in angiogenesis and inflammation. The number of placental CD3, CD68 and CD11 cells was significantly higher in patients affected by rheumatic diseases (SSc+ORD) compared with HC. Hepatocyte growth factor was significantly increased in the group of rheumatic diseases patients (SSc+ORD) compared with HC, while chemokine (C-C motif) ligand 5 (CCL5) was significantly higher in SSc patients compared with ORD and HC. CCL5 levels directly correlated with the number of all local inflammatory cells and higher levels were associated with histological villitis. Inflammatory alterations characterize placentas from rheumatic disease patients and could predispose to obstetric complications in these subjects.
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DOI：
10.1016/j.ejogrb.2006.01.016
发表时间：
2006-09-01
期刊：
EUROPEAN JOURNAL OF OBSTETRICS GYNECOLOGY AND REPRODUCTIVE BIOLOGY
影响因子：
--
作者：
Ananth, Cande V.;Oyelese, Yinka;Smulian, John C.
通讯作者：
Smulian, John C.
影响因子：
3.9
作者：
Marder W;Knight JS;Kaplan MJ;Somers EC;Zhang X;O'Dell AA;Padmanabhan V;Lieberman RW
通讯作者：
Lieberman RW
DOI：
10.1016/s0002-9378(98)70277-7
发表时间：
1998-07-01
影响因子：
9.8
作者：
Magid, MS;Kaplan, C;Lockshin, MD
通讯作者：
Lockshin, MD
影响因子：
16.8
作者：
Appay, V;Rowland-Jones, SL
通讯作者：
Rowland-Jones, SL
影响因子：
--
作者：
Lee, Eun Bong;Zhao, Jinying;Song, Yeong Wook
通讯作者：
Song, Yeong Wook